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miRNA: 3'- caCAGCGCaaUAGUCGGC-CGuuCGUG- -5'
miRNA: 3'- -CACAGC----GCaaUaGUCGGCcGUUCGUG- -5'
miRNA: 3'- cACAGCG--CAAuaGUCGGCCGuucGUG- -5'
miRNA: 3'- caCAGCGCAa--UAGU--CGGCCGU--------------UCGUg -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 8989 | 5' | -53.8 | NC_002512.2 | + | 156298 | 0.66 | 0.982603 |
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Target: 5'- -cGUCGUGaaGUCGGCCGuGUc-GCACg -3' miRNA: 3'- caCAGCGCaaUAGUCGGC-CGuuCGUG- -5' |
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| 8989 | 5' | -53.8 | NC_002512.2 | + | 18424 | 0.66 | 0.980556 |
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Target: 5'- gGUGUCGgaucCGggG-CAGCCG-CAGGCGCc -3' miRNA: 3'- -CACAGC----GCaaUaGUCGGCcGUUCGUG- -5' |
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| 8989 | 5' | -53.8 | NC_002512.2 | + | 199917 | 0.66 | 0.973338 |
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Target: 5'- cUGUCGUccGUUcgCGGCCGGCcgcCGCu -3' miRNA: 3'- cACAGCG--CAAuaGUCGGCCGuucGUG- -5' |
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| 8989 | 5' | -53.8 | NC_002512.2 | + | 104261 | 0.65 | 0.983936 |
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Target: 5'- -gGUCGUGUgugGUCGccGCCGGCGucuuucucgcguccGGCGg -3' miRNA: 3'- caCAGCGCAa--UAGU--CGGCCGU--------------UCGUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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