Results 41 - 43 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8992 | 3' | -58.2 | NC_002512.2 | + | 171849 | 0.67 | 0.855829 |
Target: 5'- -cGGUCcgGCCgCgGCCGGGAgGGgGCGg -3' miRNA: 3'- guCUAGuaCGG-G-UGGCCCUgCCaCGU- -5' |
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8992 | 3' | -58.2 | NC_002512.2 | + | 219402 | 0.67 | 0.843627 |
Target: 5'- -uGGUCG-GCCCGCCgcgacgcccacgcccGGGGCGGcgGCGg -3' miRNA: 3'- guCUAGUaCGGGUGG---------------CCCUGCCa-CGU- -5' |
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8992 | 3' | -58.2 | NC_002512.2 | + | 188200 | 1.08 | 0.003208 |
Target: 5'- gCAGAUCAUGCCCACCGGGACGGUGCAg -3' miRNA: 3'- -GUCUAGUACGGGUGGCCCUGCCACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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