Results 1 - 20 of 84 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8995 | 5' | -59.4 | NC_002512.2 | + | 126769 | 0.66 | 0.867685 |
Target: 5'- gGGGCgCGGCGgcggCGGCgaC-GCGUGcCGCg -3' miRNA: 3'- -CCCG-GUCGCa---GCUGgaGaCGCAC-GUG- -5' |
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8995 | 5' | -59.4 | NC_002512.2 | + | 189539 | 0.66 | 0.867685 |
Target: 5'- cGGUCGGCGUccgCGGCaUCUGCugGUGCGa -3' miRNA: 3'- cCCGGUCGCA---GCUGgAGACG--CACGUg -5' |
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8995 | 5' | -59.4 | NC_002512.2 | + | 95376 | 0.66 | 0.867685 |
Target: 5'- -cGCCGaCGUCGACgUCgGCGUGgUACg -3' miRNA: 3'- ccCGGUcGCAGCUGgAGaCGCAC-GUG- -5' |
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8995 | 5' | -59.4 | NC_002512.2 | + | 104953 | 0.66 | 0.867685 |
Target: 5'- cGGCgCGGCGcCGGCCUC-GUGUcucucGUACg -3' miRNA: 3'- cCCG-GUCGCaGCUGGAGaCGCA-----CGUG- -5' |
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8995 | 5' | -59.4 | NC_002512.2 | + | 90932 | 0.66 | 0.866974 |
Target: 5'- gGGGCCGGCG-CGGCCgccgccgUCgugGUGgugGUGCu -3' miRNA: 3'- -CCCGGUCGCaGCUGG-------AGa--CGCa--CGUG- -5' |
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8995 | 5' | -59.4 | NC_002512.2 | + | 18930 | 0.66 | 0.866974 |
Target: 5'- aGGGcCCGGacgaccuCGUUGGCCUCgggGCGgacUGCAUc -3' miRNA: 3'- -CCC-GGUC-------GCAGCUGGAGa--CGC---ACGUG- -5' |
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8995 | 5' | -59.4 | NC_002512.2 | + | 180746 | 0.66 | 0.860496 |
Target: 5'- aGGCCuguaccGCGUccgCGAUCUUgGCGUGCAg -3' miRNA: 3'- cCCGGu-----CGCA---GCUGGAGaCGCACGUg -5' |
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8995 | 5' | -59.4 | NC_002512.2 | + | 84539 | 0.66 | 0.860496 |
Target: 5'- -aGCCGGCGUCGGgCggcgCgGCGagGCGCu -3' miRNA: 3'- ccCGGUCGCAGCUgGa---GaCGCa-CGUG- -5' |
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8995 | 5' | -59.4 | NC_002512.2 | + | 175618 | 0.66 | 0.860496 |
Target: 5'- gGGuGCCAGgGUCGccgacgccgccGCC-CUGCGcUGCGg -3' miRNA: 3'- -CC-CGGUCgCAGC-----------UGGaGACGC-ACGUg -5' |
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8995 | 5' | -59.4 | NC_002512.2 | + | 124275 | 0.66 | 0.860496 |
Target: 5'- cGG-CGGCGUCgGACCcggGCGUGCGg -3' miRNA: 3'- cCCgGUCGCAG-CUGGagaCGCACGUg -5' |
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8995 | 5' | -59.4 | NC_002512.2 | + | 117463 | 0.66 | 0.860496 |
Target: 5'- -cGCCGGUGUCGAUCgcgCUGCucaGCAa -3' miRNA: 3'- ccCGGUCGCAGCUGGa--GACGca-CGUg -5' |
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8995 | 5' | -59.4 | NC_002512.2 | + | 198741 | 0.66 | 0.859767 |
Target: 5'- cGGGCCAgguccGCGaagaCGGCCUucgucucggggacCUGgGUGUACg -3' miRNA: 3'- -CCCGGU-----CGCa---GCUGGA-------------GACgCACGUG- -5' |
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8995 | 5' | -59.4 | NC_002512.2 | + | 91365 | 0.66 | 0.853121 |
Target: 5'- cGGGCUGGaCGacgCGACCcugaagacgCUGCG-GCGCu -3' miRNA: 3'- -CCCGGUC-GCa--GCUGGa--------GACGCaCGUG- -5' |
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8995 | 5' | -59.4 | NC_002512.2 | + | 102254 | 0.66 | 0.853121 |
Target: 5'- cGGGCCcggguccgGGCGUCGuccAUCUCgGCucgGCGCg -3' miRNA: 3'- -CCCGG--------UCGCAGC---UGGAGaCGca-CGUG- -5' |
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8995 | 5' | -59.4 | NC_002512.2 | + | 10606 | 0.66 | 0.85312 |
Target: 5'- cGGGCCacGGCGUCGGCgUCcGCccccUGC-Cg -3' miRNA: 3'- -CCCGG--UCGCAGCUGgAGaCGc---ACGuG- -5' |
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8995 | 5' | -59.4 | NC_002512.2 | + | 63943 | 0.66 | 0.85312 |
Target: 5'- cGGCCGGCGUCcuucugguaGACCcgacacaUGCgGUGCAg -3' miRNA: 3'- cCCGGUCGCAG---------CUGGag-----ACG-CACGUg -5' |
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8995 | 5' | -59.4 | NC_002512.2 | + | 67739 | 0.66 | 0.85312 |
Target: 5'- cGGUCGGCGUgcCGGCCUC-GCGaGC-Cg -3' miRNA: 3'- cCCGGUCGCA--GCUGGAGaCGCaCGuG- -5' |
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8995 | 5' | -59.4 | NC_002512.2 | + | 126974 | 0.66 | 0.85312 |
Target: 5'- gGGGCCcguGGcCGUCGACCgc-GCcagGCGCa -3' miRNA: 3'- -CCCGG---UC-GCAGCUGGagaCGca-CGUG- -5' |
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8995 | 5' | -59.4 | NC_002512.2 | + | 114997 | 0.66 | 0.846327 |
Target: 5'- gGGGCUGGUGgacaccuuccuccaGACCcugCUGCG-GCACg -3' miRNA: 3'- -CCCGGUCGCag------------CUGGa--GACGCaCGUG- -5' |
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8995 | 5' | -59.4 | NC_002512.2 | + | 198163 | 0.66 | 0.845563 |
Target: 5'- cGGGUCGGCGcCGGCCg--GCGgaccucCACg -3' miRNA: 3'- -CCCGGUCGCaGCUGGagaCGCac----GUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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