Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
90 | 5' | -60.4 | AC_000006.1 | + | 14850 | 0.66 | 0.346668 |
Target: 5'- aUCCUCu--GC-CGUcCGCCCgCGCCCa -3' miRNA: 3'- gAGGAGcguCGaGUA-GUGGGgGCGGG- -5' |
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90 | 5' | -60.4 | AC_000006.1 | + | 27401 | 0.66 | 0.346668 |
Target: 5'- aUUCUCGgGggacccagaGCUgGUCGCCCuuGCUCg -3' miRNA: 3'- gAGGAGCgU---------CGAgUAGUGGGggCGGG- -5' |
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90 | 5' | -60.4 | AC_000006.1 | + | 30942 | 0.66 | 0.338439 |
Target: 5'- uCUCCUCcucaAGgaCAUCAUCCagcaccaCGCCCa -3' miRNA: 3'- -GAGGAGcg--UCgaGUAGUGGGg------GCGGG- -5' |
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90 | 5' | -60.4 | AC_000006.1 | + | 23065 | 0.66 | 0.314609 |
Target: 5'- uCUUCUCGCcGCgCGU-GCCCCCcaaacgccaGCCCa -3' miRNA: 3'- -GAGGAGCGuCGaGUAgUGGGGG---------CGGG- -5' |
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90 | 5' | -60.4 | AC_000006.1 | + | 23452 | 0.67 | 0.306952 |
Target: 5'- -aCUUCGCcuaccccGC-CGUCaACCuCCCGCCCa -3' miRNA: 3'- gaGGAGCGu------CGaGUAG-UGG-GGGCGGG- -5' |
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90 | 5' | -60.4 | AC_000006.1 | + | 6255 | 0.67 | 0.306952 |
Target: 5'- -aCCUCGCcgcgcaggGGCUCGUUAguccagcagaguCUgCCGCCCu -3' miRNA: 3'- gaGGAGCG--------UCGAGUAGU------------GGgGGCGGG- -5' |
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90 | 5' | -60.4 | AC_000006.1 | + | 26578 | 0.67 | 0.306952 |
Target: 5'- uUCCuUCGCAGuCUCcaaCGCCUgCGCCa -3' miRNA: 3'- gAGG-AGCGUC-GAGua-GUGGGgGCGGg -5' |
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90 | 5' | -60.4 | AC_000006.1 | + | 21407 | 0.67 | 0.299439 |
Target: 5'- gCUCCacauaCGcCGGCUCAUCugCagggCGCCCa -3' miRNA: 3'- -GAGGa----GC-GUCGAGUAGugGgg--GCGGG- -5' |
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90 | 5' | -60.4 | AC_000006.1 | + | 12428 | 0.67 | 0.29207 |
Target: 5'- -cCCUCGCcGC-CGcCACCUCCucuGCCCa -3' miRNA: 3'- gaGGAGCGuCGaGUaGUGGGGG---CGGG- -5' |
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90 | 5' | -60.4 | AC_000006.1 | + | 22488 | 0.67 | 0.284842 |
Target: 5'- uCUCCUgGaaggucuGCUCGUcCACCUgCGCCUg -3' miRNA: 3'- -GAGGAgCgu-----CGAGUA-GUGGGgGCGGG- -5' |
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90 | 5' | -60.4 | AC_000006.1 | + | 24160 | 0.67 | 0.284842 |
Target: 5'- -cCCUCGCgcAGCUCc-CugC-CCGCCCg -3' miRNA: 3'- gaGGAGCG--UCGAGuaGugGgGGCGGG- -5' |
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90 | 5' | -60.4 | AC_000006.1 | + | 28541 | 0.67 | 0.270813 |
Target: 5'- --aUUUGCGGCaUGUaCGCCUCCGCCCu -3' miRNA: 3'- gagGAGCGUCGaGUA-GUGGGGGCGGG- -5' |
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90 | 5' | -60.4 | AC_000006.1 | + | 13735 | 0.68 | 0.244429 |
Target: 5'- uUCCUgaccacgguggUGCAGaaCAacgauuUCACCCCCGCCg -3' miRNA: 3'- gAGGA-----------GCGUCgaGU------AGUGGGGGCGGg -5' |
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90 | 5' | -60.4 | AC_000006.1 | + | 18926 | 0.69 | 0.214491 |
Target: 5'- aUCC-CGCGGCUCggCAgCagcaCCGCCUg -3' miRNA: 3'- gAGGaGCGUCGAGuaGUgGg---GGCGGG- -5' |
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90 | 5' | -60.4 | AC_000006.1 | + | 29650 | 0.7 | 0.192857 |
Target: 5'- -gCCUUugcgGCAGUUUcgCACgCCUCGCCCg -3' miRNA: 3'- gaGGAG----CGUCGAGuaGUG-GGGGCGGG- -5' |
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90 | 5' | -60.4 | AC_000006.1 | + | 27759 | 0.7 | 0.182773 |
Target: 5'- gUCCUCGCcgcccAGgUCAUCaacaaccgaGCCUCCGUCCc -3' miRNA: 3'- gAGGAGCG-----UCgAGUAG---------UGGGGGCGGG- -5' |
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90 | 5' | -60.4 | AC_000006.1 | + | 14890 | 0.71 | 0.151072 |
Target: 5'- gUCCUUGUAGUugaucUCAUCgACCaCCUGCCUg -3' miRNA: 3'- gAGGAGCGUCG-----AGUAG-UGG-GGGCGGG- -5' |
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90 | 5' | -60.4 | AC_000006.1 | + | 16581 | 0.72 | 0.13908 |
Target: 5'- -aCC-CGCcGC-CGUCugcCCCCCGCCCg -3' miRNA: 3'- gaGGaGCGuCGaGUAGu--GGGGGCGGG- -5' |
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90 | 5' | -60.4 | AC_000006.1 | + | 27008 | 0.73 | 0.114424 |
Target: 5'- gCUCCcgCGCgGGCUCGUCAgccUCUCCGUCCu -3' miRNA: 3'- -GAGGa-GCG-UCGAGUAGU---GGGGGCGGG- -5' |
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90 | 5' | -60.4 | AC_000006.1 | + | 25168 | 0.73 | 0.111254 |
Target: 5'- cCUUCUCGCGGCUCAggUACUCCgaggucgugcaGCCCg -3' miRNA: 3'- -GAGGAGCGUCGAGUa-GUGGGGg----------CGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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