Results 1 - 20 of 82 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9000 | 3' | -59.1 | NC_002512.2 | + | 147487 | 0.65 | 0.899339 |
Target: 5'- -gGCGCUGCgGA---CGGGCGcgaccggcgcuucUCCCGu -3' miRNA: 3'- gaCGCGAUGgCUaagGCCCGC-------------AGGGC- -5' |
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9000 | 3' | -59.1 | NC_002512.2 | + | 80420 | 0.66 | 0.893784 |
Target: 5'- -gGCGUgGCUG--UCgGcGGCGUCCCGg -3' miRNA: 3'- gaCGCGaUGGCuaAGgC-CCGCAGGGC- -5' |
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9000 | 3' | -59.1 | NC_002512.2 | + | 209947 | 0.66 | 0.893784 |
Target: 5'- -cGUGCUGCuCGA--CCGGGCGcgggUCCUc -3' miRNA: 3'- gaCGCGAUG-GCUaaGGCCCGC----AGGGc -5' |
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9000 | 3' | -59.1 | NC_002512.2 | + | 172822 | 0.66 | 0.893784 |
Target: 5'- gUGCGcCUACCGuucgCCcGGCGcCCCc -3' miRNA: 3'- gACGC-GAUGGCuaa-GGcCCGCaGGGc -5' |
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9000 | 3' | -59.1 | NC_002512.2 | + | 211431 | 0.66 | 0.887417 |
Target: 5'- -cGCGCUGCCcuucugcccgGAgcUCGGGCG-CCUGc -3' miRNA: 3'- gaCGCGAUGG----------CUaaGGCCCGCaGGGC- -5' |
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9000 | 3' | -59.1 | NC_002512.2 | + | 136549 | 0.66 | 0.887417 |
Target: 5'- aUGCGCacgcagcucuUGCgGAUgaUCCGGGUcagCCCGg -3' miRNA: 3'- gACGCG----------AUGgCUA--AGGCCCGca-GGGC- -5' |
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9000 | 3' | -59.1 | NC_002512.2 | + | 15874 | 0.66 | 0.880849 |
Target: 5'- -cGCacCUAUCGccUCCGGGCGUCgCGc -3' miRNA: 3'- gaCGc-GAUGGCuaAGGCCCGCAGgGC- -5' |
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9000 | 3' | -59.1 | NC_002512.2 | + | 20069 | 0.66 | 0.880849 |
Target: 5'- aCUGgGCcagACCGAgUCCGGucuCGUCgCCGa -3' miRNA: 3'- -GACgCGa--UGGCUaAGGCCc--GCAG-GGC- -5' |
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9000 | 3' | -59.1 | NC_002512.2 | + | 88692 | 0.66 | 0.880849 |
Target: 5'- gCUGCGCUGCUGcAUgaUCCGGuaGCGggCCa -3' miRNA: 3'- -GACGCGAUGGC-UA--AGGCC--CGCagGGc -5' |
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9000 | 3' | -59.1 | NC_002512.2 | + | 101803 | 0.66 | 0.880849 |
Target: 5'- -aGCGCUuCCGAggaggcUUCCuGGGCG-CCgCGa -3' miRNA: 3'- gaCGCGAuGGCU------AAGG-CCCGCaGG-GC- -5' |
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9000 | 3' | -59.1 | NC_002512.2 | + | 115719 | 0.66 | 0.878166 |
Target: 5'- -gGCGCcACCGAggaggaccgggcCCGGGuCGUCCUc -3' miRNA: 3'- gaCGCGaUGGCUaa----------GGCCC-GCAGGGc -5' |
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9000 | 3' | -59.1 | NC_002512.2 | + | 103395 | 0.66 | 0.874083 |
Target: 5'- -cGCGCccaacGCCGGggaUCCGGGCGaCgCGa -3' miRNA: 3'- gaCGCGa----UGGCUa--AGGCCCGCaGgGC- -5' |
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9000 | 3' | -59.1 | NC_002512.2 | + | 70130 | 0.66 | 0.874083 |
Target: 5'- -aGCGUgACCGAcUCCGGGCacgaGUCUa- -3' miRNA: 3'- gaCGCGaUGGCUaAGGCCCG----CAGGgc -5' |
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9000 | 3' | -59.1 | NC_002512.2 | + | 69781 | 0.66 | 0.874083 |
Target: 5'- cCUGCuCcACCG--UCUGGGCGUCCg- -3' miRNA: 3'- -GACGcGaUGGCuaAGGCCCGCAGGgc -5' |
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9000 | 3' | -59.1 | NC_002512.2 | + | 135126 | 0.66 | 0.867124 |
Target: 5'- -gGCGCgacgcuCCGAgggCCGGGcCGcCCUGg -3' miRNA: 3'- gaCGCGau----GGCUaa-GGCCC-GCaGGGC- -5' |
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9000 | 3' | -59.1 | NC_002512.2 | + | 224611 | 0.66 | 0.867124 |
Target: 5'- -cGCGgUGCCGA--CCGGGCucgggGUCgCCGc -3' miRNA: 3'- gaCGCgAUGGCUaaGGCCCG-----CAG-GGC- -5' |
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9000 | 3' | -59.1 | NC_002512.2 | + | 159360 | 0.66 | 0.867124 |
Target: 5'- -gGCGU--CgGggUCgGGGCGUCCCa -3' miRNA: 3'- gaCGCGauGgCuaAGgCCCGCAGGGc -5' |
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9000 | 3' | -59.1 | NC_002512.2 | + | 138051 | 0.66 | 0.867124 |
Target: 5'- cCUGCGggACCGGaUCCGGGUGa--CGa -3' miRNA: 3'- -GACGCgaUGGCUaAGGCCCGCaggGC- -5' |
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9000 | 3' | -59.1 | NC_002512.2 | + | 221512 | 0.66 | 0.867124 |
Target: 5'- cCUGCGCggcGCCGcggaUCCucGGCGcUCCCGc -3' miRNA: 3'- -GACGCGa--UGGCua--AGGc-CCGC-AGGGC- -5' |
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9000 | 3' | -59.1 | NC_002512.2 | + | 51055 | 0.66 | 0.867123 |
Target: 5'- cCUGCGac-CCGAgcacgUCCGGGCGcUgUCGg -3' miRNA: 3'- -GACGCgauGGCUa----AGGCCCGC-AgGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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