Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9003 | 5' | -51.7 | NC_002512.2 | + | 181842 | 1.09 | 0.012098 |
Target: 5'- gCGUGGUGGAACAGGAACGUGUUGCUGu -3' miRNA: 3'- -GCACCACCUUGUCCUUGCACAACGAC- -5' |
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9003 | 5' | -51.7 | NC_002512.2 | + | 194810 | 0.78 | 0.651291 |
Target: 5'- cCG-GGUGGAGCAGGAGCucggcaccGUGgUGCUGu -3' miRNA: 3'- -GCaCCACCUUGUCCUUG--------CACaACGAC- -5' |
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9003 | 5' | -51.7 | NC_002512.2 | + | 81981 | 0.71 | 0.928899 |
Target: 5'- gGUGGUGGcGGCGGGGGCGUcucgGggGCg- -3' miRNA: 3'- gCACCACC-UUGUCCUUGCA----CaaCGac -5' |
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9003 | 5' | -51.7 | NC_002512.2 | + | 228059 | 0.7 | 0.947949 |
Target: 5'- gGUGGUGGcAGCAGGggUGgggGgaGCg- -3' miRNA: 3'- gCACCACC-UUGUCCuuGCa--CaaCGac -5' |
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9003 | 5' | -51.7 | NC_002512.2 | + | 147053 | 0.7 | 0.963272 |
Target: 5'- gCGcGGaccGGGGCGGGAACGUGUggGCg- -3' miRNA: 3'- -GCaCCa--CCUUGUCCUUGCACAa-CGac -5' |
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9003 | 5' | -51.7 | NC_002512.2 | + | 67174 | 0.69 | 0.975128 |
Target: 5'- -uUGGUGGAgacgaaGCGGGAGC-UGcUGCUGc -3' miRNA: 3'- gcACCACCU------UGUCCUUGcACaACGAC- -5' |
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9003 | 5' | -51.7 | NC_002512.2 | + | 26221 | 0.69 | 0.977595 |
Target: 5'- cCGUGGcgacGGAGaCGGGAccgguauggACGUGcUGCUGg -3' miRNA: 3'- -GCACCa---CCUU-GUCCU---------UGCACaACGAC- -5' |
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9003 | 5' | -51.7 | NC_002512.2 | + | 82352 | 0.67 | 0.991327 |
Target: 5'- --cGGUGGGcguGCGGGGGCGgcggaggGUgaUGCUGc -3' miRNA: 3'- gcaCCACCU---UGUCCUUGCa------CA--ACGAC- -5' |
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9003 | 5' | -51.7 | NC_002512.2 | + | 191082 | 0.67 | 0.990118 |
Target: 5'- uGUGcggcaaGUGGGGguGGAugGUggGUUGCUGc -3' miRNA: 3'- gCAC------CACCUUguCCUugCA--CAACGAC- -5' |
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9003 | 5' | -51.7 | NC_002512.2 | + | 176486 | 0.66 | 0.996346 |
Target: 5'- ----uUGGGACGGGGACGUGggGUc- -3' miRNA: 3'- gcaccACCUUGUCCUUGCACaaCGac -5' |
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9003 | 5' | -51.7 | NC_002512.2 | + | 160118 | 0.66 | 0.995046 |
Target: 5'- --cGGUGGAGCAGGAccuggGCGagGUgGCg- -3' miRNA: 3'- gcaCCACCUUGUCCU-----UGCa-CAaCGac -5' |
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9003 | 5' | -51.7 | NC_002512.2 | + | 121810 | 0.66 | 0.995046 |
Target: 5'- aCGUGGUGGAcagcacGCAcGGGGCGgGUgacCUGa -3' miRNA: 3'- -GCACCACCU------UGU-CCUUGCaCAac-GAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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