Results 1 - 20 of 73 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9004 | 5' | -66.3 | NC_002512.2 | + | 223532 | 0.66 | 0.550079 |
Target: 5'- ---cGGCacgcGCCcgcGGCCCGCGgCCCCGc- -3' miRNA: 3'- gugaCCGa---CGG---CCGGGCGC-GGGGCau -5' |
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9004 | 5' | -66.3 | NC_002512.2 | + | 54099 | 0.66 | 0.550079 |
Target: 5'- cCGCcaaGCUGUCGGCCUGCGacgugaccaCCCCGc- -3' miRNA: 3'- -GUGac-CGACGGCCGGGCGC---------GGGGCau -5' |
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9004 | 5' | -66.3 | NC_002512.2 | + | 131752 | 0.66 | 0.550079 |
Target: 5'- gGCgGGCgGCCGcGacgaCCaGCGCCCCGg- -3' miRNA: 3'- gUGaCCGaCGGC-Cg---GG-CGCGGGGCau -5' |
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9004 | 5' | -66.3 | NC_002512.2 | + | 211729 | 0.66 | 0.531769 |
Target: 5'- aGCUGuGCcucgGCCuGGCCCGCGacgcggCCCGg- -3' miRNA: 3'- gUGAC-CGa---CGG-CCGGGCGCg-----GGGCau -5' |
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9004 | 5' | -66.3 | NC_002512.2 | + | 123389 | 0.66 | 0.531769 |
Target: 5'- cCGCUGGacgcgaagaucCUGCCgucGGCCCuG-GCCCCGUu -3' miRNA: 3'- -GUGACC-----------GACGG---CCGGG-CgCGGGGCAu -5' |
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9004 | 5' | -66.3 | NC_002512.2 | + | 67736 | 0.66 | 0.531769 |
Target: 5'- uCGCggucGGCgUGCCGGCCuCGCGagCCGg- -3' miRNA: 3'- -GUGa---CCG-ACGGCCGG-GCGCggGGCau -5' |
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9004 | 5' | -66.3 | NC_002512.2 | + | 82312 | 0.66 | 0.531769 |
Target: 5'- gCGCUGGgUGUagaGGgCCGCgggccGCCCCGg- -3' miRNA: 3'- -GUGACCgACGg--CCgGGCG-----CGGGGCau -5' |
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9004 | 5' | -66.3 | NC_002512.2 | + | 114201 | 0.66 | 0.531769 |
Target: 5'- cCACcGuGCUGuaggcCCGGCCCGaGCCCaCGUGc -3' miRNA: 3'- -GUGaC-CGAC-----GGCCGGGCgCGGG-GCAU- -5' |
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9004 | 5' | -66.3 | NC_002512.2 | + | 117724 | 0.66 | 0.531769 |
Target: 5'- uGCgGGCUcgagcGCCGGCUCGCcgucgaccGCCUCGUc -3' miRNA: 3'- gUGaCCGA-----CGGCCGGGCG--------CGGGGCAu -5' |
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9004 | 5' | -66.3 | NC_002512.2 | + | 114802 | 0.66 | 0.531769 |
Target: 5'- aCGCgGGUgGCCaGGCgCCGCGCgacguuCCCGUGu -3' miRNA: 3'- -GUGaCCGaCGG-CCG-GGCGCG------GGGCAU- -5' |
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9004 | 5' | -66.3 | NC_002512.2 | + | 149157 | 0.66 | 0.530859 |
Target: 5'- cCGCggGGCcggUGCCgcccgcgGGCCCGCGCgCCCu-- -3' miRNA: 3'- -GUGa-CCG---ACGG-------CCGGGCGCG-GGGcau -5' |
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9004 | 5' | -66.3 | NC_002512.2 | + | 5264 | 0.66 | 0.528133 |
Target: 5'- cCGCggcGGCgaccccgagcccgGUCGGCaCCGCGUCCCGc- -3' miRNA: 3'- -GUGa--CCGa------------CGGCCG-GGCGCGGGGCau -5' |
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9004 | 5' | -66.3 | NC_002512.2 | + | 188782 | 0.66 | 0.522696 |
Target: 5'- gGCggcGGCU-CCGGCCUcgGCGgCCCCGa- -3' miRNA: 3'- gUGa--CCGAcGGCCGGG--CGC-GGGGCau -5' |
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9004 | 5' | -66.3 | NC_002512.2 | + | 214235 | 0.66 | 0.522696 |
Target: 5'- gGC-GGCUcGUCGGCUCGCcGCCCgCGg- -3' miRNA: 3'- gUGaCCGA-CGGCCGGGCG-CGGG-GCau -5' |
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9004 | 5' | -66.3 | NC_002512.2 | + | 90915 | 0.66 | 0.519084 |
Target: 5'- gACUGGCggccgcgaacgggGCCGGCgCGgcCGCCgCCGUc -3' miRNA: 3'- gUGACCGa------------CGGCCGgGC--GCGG-GGCAu -5' |
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9004 | 5' | -66.3 | NC_002512.2 | + | 58197 | 0.66 | 0.513684 |
Target: 5'- aCGCUGGUgaguggGCgCGGCgCGCaCCCCGc- -3' miRNA: 3'- -GUGACCGa-----CG-GCCGgGCGcGGGGCau -5' |
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9004 | 5' | -66.3 | NC_002512.2 | + | 13322 | 0.66 | 0.513684 |
Target: 5'- uCGCUGGCccgccgcaUGCUGGCguUCGCGCgCCGc- -3' miRNA: 3'- -GUGACCG--------ACGGCCG--GGCGCGgGGCau -5' |
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9004 | 5' | -66.3 | NC_002512.2 | + | 219207 | 0.66 | 0.513684 |
Target: 5'- cCGCUGcaCUGCCGGgCCG-GCCUCGg- -3' miRNA: 3'- -GUGACc-GACGGCCgGGCgCGGGGCau -5' |
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9004 | 5' | -66.3 | NC_002512.2 | + | 157356 | 0.66 | 0.513684 |
Target: 5'- -cCUGGCcGCCgaGGUCCGgGCCgCCGg- -3' miRNA: 3'- guGACCGaCGG--CCGGGCgCGG-GGCau -5' |
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9004 | 5' | -66.3 | NC_002512.2 | + | 116732 | 0.66 | 0.504736 |
Target: 5'- cCGCccGGCcgcgGCgaGGCCgGCGCCCCGc- -3' miRNA: 3'- -GUGa-CCGa---CGg-CCGGgCGCGGGGCau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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