Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9019 | 3' | -60.1 | NC_002512.2 | + | 221291 | 0.66 | 0.820668 |
Target: 5'- gGGGGgggaGGcCGGGGUcGGCGGGGAGc -3' miRNA: 3'- -CUCCaggaCCaGUUCCA-CCGCCCCUU- -5' |
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9019 | 3' | -60.1 | NC_002512.2 | + | 189706 | 0.66 | 0.820668 |
Target: 5'- cGAcGUCCgggGGaugUCGGGGcGGUGGGGAGg -3' miRNA: 3'- -CUcCAGGa--CC---AGUUCCaCCGCCCCUU- -5' |
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9019 | 3' | -60.1 | NC_002512.2 | + | 4634 | 0.66 | 0.795277 |
Target: 5'- -uGGUgaCCUGGac--GGUGGUGGGGAu -3' miRNA: 3'- cuCCA--GGACCaguuCCACCGCCCCUu -5' |
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9019 | 3' | -60.1 | NC_002512.2 | + | 217718 | 0.66 | 0.786532 |
Target: 5'- cGAGGUCCgcuUCAGGGgcGGCaGGGAc -3' miRNA: 3'- -CUCCAGGaccAGUUCCa-CCGcCCCUu -5' |
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9019 | 3' | -60.1 | NC_002512.2 | + | 213815 | 0.66 | 0.786532 |
Target: 5'- gGAGGguucCCUGGacgagagucUCGAGGcgcgGcGCGGGGAGa -3' miRNA: 3'- -CUCCa---GGACC---------AGUUCCa---C-CGCCCCUU- -5' |
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9019 | 3' | -60.1 | NC_002512.2 | + | 24696 | 0.66 | 0.777658 |
Target: 5'- gGGGGUCUc-GUCGAGGacgcgGGCGaGGGAGa -3' miRNA: 3'- -CUCCAGGacCAGUUCCa----CCGC-CCCUU- -5' |
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9019 | 3' | -60.1 | NC_002512.2 | + | 86243 | 0.66 | 0.777658 |
Target: 5'- gGAGGaCCcgGGggaGAGGgccgaGGCGGGGAGg -3' miRNA: 3'- -CUCCaGGa-CCag-UUCCa----CCGCCCCUU- -5' |
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9019 | 3' | -60.1 | NC_002512.2 | + | 146838 | 0.66 | 0.777658 |
Target: 5'- cGAGGUcgCCUGGccCAGGGUGcucgagcucgcGCGGGGc- -3' miRNA: 3'- -CUCCA--GGACCa-GUUCCAC-----------CGCCCCuu -5' |
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9019 | 3' | -60.1 | NC_002512.2 | + | 75027 | 0.67 | 0.760472 |
Target: 5'- cGAGGgCCggGGaCGAGGggacgcacgacggagGGCGGGGGAg -3' miRNA: 3'- -CUCCaGGa-CCaGUUCCa--------------CCGCCCCUU- -5' |
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9019 | 3' | -60.1 | NC_002512.2 | + | 108390 | 0.67 | 0.759557 |
Target: 5'- gGGGGgcgagCCcGGU--GGGUGGgGGGGAGg -3' miRNA: 3'- -CUCCa----GGaCCAguUCCACCgCCCCUU- -5' |
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9019 | 3' | -60.1 | NC_002512.2 | + | 89190 | 0.67 | 0.722172 |
Target: 5'- cGAGGUCCaguUGGaggcguugUCgAAGGUGGCcGGGAu -3' miRNA: 3'- -CUCCAGG---ACC--------AG-UUCCACCGcCCCUu -5' |
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9019 | 3' | -60.1 | NC_002512.2 | + | 160126 | 0.67 | 0.722172 |
Target: 5'- cAGGaCCUGGgCGAGGUGGCGauccGGGc- -3' miRNA: 3'- cUCCaGGACCaGUUCCACCGC----CCCuu -5' |
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9019 | 3' | -60.1 | NC_002512.2 | + | 88552 | 0.68 | 0.683645 |
Target: 5'- aGGGUCgaGGUgGAGGUGGagauaGuGGGAGa -3' miRNA: 3'- cUCCAGgaCCAgUUCCACCg----C-CCCUU- -5' |
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9019 | 3' | -60.1 | NC_002512.2 | + | 84170 | 0.73 | 0.411485 |
Target: 5'- gGAGGUCCcGGUCcAGGUucGGCGGGa-- -3' miRNA: 3'- -CUCCAGGaCCAGuUCCA--CCGCCCcuu -5' |
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9019 | 3' | -60.1 | NC_002512.2 | + | 204907 | 0.78 | 0.206659 |
Target: 5'- -cGGUCCUGGUC-GGGUGaCGGGGAc -3' miRNA: 3'- cuCCAGGACCAGuUCCACcGCCCCUu -5' |
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9019 | 3' | -60.1 | NC_002512.2 | + | 167253 | 1.06 | 0.002735 |
Target: 5'- aGAGGUCCUGGUCAAGGUGGCGGGGAAg -3' miRNA: 3'- -CUCCAGGACCAGUUCCACCGCCCCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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