Results 1 - 20 of 33 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9070 | 3' | -55.1 | NC_002512.2 | + | 31295 | 0.66 | 0.973864 |
Target: 5'- gUCAGCgUgaacacaUCGGGAGGUGAAGacGUcUCACa -3' miRNA: 3'- -GGUCGgA-------GGUCCUCCACUUC--CA-AGUG- -5' |
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9070 | 3' | -55.1 | NC_002512.2 | + | 86220 | 0.66 | 0.968577 |
Target: 5'- cCCGGCUUCgGGGAGGgggacgaGGAGGa---- -3' miRNA: 3'- -GGUCGGAGgUCCUCCa------CUUCCaagug -5' |
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9070 | 3' | -55.1 | NC_002512.2 | + | 132483 | 0.66 | 0.967374 |
Target: 5'- gCCAGCC-CCAGGAGGaacugcgUCAUg -3' miRNA: 3'- -GGUCGGaGGUCCUCCacuuccaAGUG- -5' |
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9070 | 3' | -55.1 | NC_002512.2 | + | 206093 | 0.66 | 0.96519 |
Target: 5'- gCAGCUgcaggUUCGGGAGGUGGgacaccaGGGUcaggCGCa -3' miRNA: 3'- gGUCGG-----AGGUCCUCCACU-------UCCAa---GUG- -5' |
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9070 | 3' | -55.1 | NC_002512.2 | + | 21542 | 0.66 | 0.962234 |
Target: 5'- aCCuG-CUCCucGGGGGUGAGGGUgUCGu -3' miRNA: 3'- -GGuCgGAGGu-CCUCCACUUCCA-AGUg -5' |
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9070 | 3' | -55.1 | NC_002512.2 | + | 86160 | 0.66 | 0.95839 |
Target: 5'- gCC-GCCUCCgaggcccugcugcGGGAGcUGGAGGU-CGCg -3' miRNA: 3'- -GGuCGGAGG-------------UCCUCcACUUCCAaGUG- -5' |
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9070 | 3' | -55.1 | NC_002512.2 | + | 174255 | 0.67 | 0.955052 |
Target: 5'- cCCucGCCcgggUCCGGGucccgGGGUcucGggGGUUCGCg -3' miRNA: 3'- -GGu-CGG----AGGUCC-----UCCA---CuuCCAAGUG- -5' |
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9070 | 3' | -55.1 | NC_002512.2 | + | 57274 | 0.67 | 0.955052 |
Target: 5'- cUCAGCCUCCGGuGAuagaccgGGAGGU-CGCa -3' miRNA: 3'- -GGUCGGAGGUC-CUcca----CUUCCAaGUG- -5' |
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9070 | 3' | -55.1 | NC_002512.2 | + | 199068 | 0.67 | 0.951135 |
Target: 5'- cUCAGCCguugguagUCCAGGcccgAGGUGGccgccAGGUcCACg -3' miRNA: 3'- -GGUCGG--------AGGUCC----UCCACU-----UCCAaGUG- -5' |
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9070 | 3' | -55.1 | NC_002512.2 | + | 3989 | 0.67 | 0.946997 |
Target: 5'- --cGCgCUCCucGGGAGGcGggGGgcgUCGCa -3' miRNA: 3'- gguCG-GAGG--UCCUCCaCuuCCa--AGUG- -5' |
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9070 | 3' | -55.1 | NC_002512.2 | + | 159878 | 0.67 | 0.942633 |
Target: 5'- aCCGGaCg-CAGGGGGUGGAGGUgugUCGa -3' miRNA: 3'- -GGUCgGagGUCCUCCACUUCCA---AGUg -5' |
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9070 | 3' | -55.1 | NC_002512.2 | + | 91294 | 0.67 | 0.942633 |
Target: 5'- aCCcGCCUgaAGGAGGUGucGGcggCGCa -3' miRNA: 3'- -GGuCGGAggUCCUCCACuuCCaa-GUG- -5' |
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9070 | 3' | -55.1 | NC_002512.2 | + | 108437 | 0.67 | 0.938043 |
Target: 5'- -gGGCCgggggCCGGGGGGUcccuccccgggcGGcgccGGGUUCGCg -3' miRNA: 3'- ggUCGGa----GGUCCUCCA------------CU----UCCAAGUG- -5' |
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9070 | 3' | -55.1 | NC_002512.2 | + | 108237 | 0.68 | 0.933223 |
Target: 5'- gCCAGUCUCCccagcAGGAucuuGGUGAGGccgCACg -3' miRNA: 3'- -GGUCGGAGG-----UCCU----CCACUUCcaaGUG- -5' |
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9070 | 3' | -55.1 | NC_002512.2 | + | 20610 | 0.68 | 0.928174 |
Target: 5'- gCAGCgCUCCacguccucgaAGGAGGUcc-GGUUCGCc -3' miRNA: 3'- gGUCG-GAGG----------UCCUCCAcuuCCAAGUG- -5' |
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9070 | 3' | -55.1 | NC_002512.2 | + | 16017 | 0.68 | 0.928174 |
Target: 5'- gUCGGCCgCCGGGuGGgGAAGGgcaGCg -3' miRNA: 3'- -GGUCGGaGGUCCuCCaCUUCCaagUG- -5' |
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9070 | 3' | -55.1 | NC_002512.2 | + | 199188 | 0.68 | 0.922895 |
Target: 5'- aCGGCCUCCAuGAGGUcGuacGGGUUguCg -3' miRNA: 3'- gGUCGGAGGUcCUCCA-Cu--UCCAAguG- -5' |
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9070 | 3' | -55.1 | NC_002512.2 | + | 223041 | 0.68 | 0.911647 |
Target: 5'- uCCGGCgaggUCCGGGAGGUGccccuGG-UCGCc -3' miRNA: 3'- -GGUCGg---AGGUCCUCCACuu---CCaAGUG- -5' |
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9070 | 3' | -55.1 | NC_002512.2 | + | 181264 | 0.68 | 0.90568 |
Target: 5'- aCCAGUC-CCAGGucGGUGu-GGcUCACg -3' miRNA: 3'- -GGUCGGaGGUCCu-CCACuuCCaAGUG- -5' |
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9070 | 3' | -55.1 | NC_002512.2 | + | 95901 | 0.69 | 0.897586 |
Target: 5'- cCCGGCCgggugCCGacgaggaccucgauGGGGGUGAAGGgaCGg -3' miRNA: 3'- -GGUCGGa----GGU--------------CCUCCACUUCCaaGUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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