Results 1 - 20 of 113 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9081 | 5' | -56.3 | NC_002512.2 | + | 99046 | 0.66 | 0.966977 |
Target: 5'- gCCCGAc-CGCCGguacUCGACcgCGGGGUu -3' miRNA: 3'- -GGGCUuaGCGGCa---GGCUGa-GCUCCAu -5' |
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9081 | 5' | -56.3 | NC_002512.2 | + | 76464 | 0.66 | 0.966977 |
Target: 5'- gCCGc--UGCCGuUCCGACagUCGAGGa- -3' miRNA: 3'- gGGCuuaGCGGC-AGGCUG--AGCUCCau -5' |
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9081 | 5' | -56.3 | NC_002512.2 | + | 123161 | 0.66 | 0.966977 |
Target: 5'- gCCGcGAUCGCCG-CCGAggcCGAGGc- -3' miRNA: 3'- gGGC-UUAGCGGCaGGCUga-GCUCCau -5' |
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9081 | 5' | -56.3 | NC_002512.2 | + | 199481 | 0.66 | 0.966977 |
Target: 5'- gCCCGcGUCcCCGUCCGucaggGCcgCGAGGa- -3' miRNA: 3'- -GGGCuUAGcGGCAGGC-----UGa-GCUCCau -5' |
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9081 | 5' | -56.3 | NC_002512.2 | + | 158679 | 0.66 | 0.963878 |
Target: 5'- gCCGGAUCaugGCCGgCCGGgaCGGGGa- -3' miRNA: 3'- gGGCUUAG---CGGCaGGCUgaGCUCCau -5' |
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9081 | 5' | -56.3 | NC_002512.2 | + | 45981 | 0.66 | 0.963878 |
Target: 5'- uCCCGAGgcaGCCGUUCGACUguccguaacCGGaaacGGUGu -3' miRNA: 3'- -GGGCUUag-CGGCAGGCUGA---------GCU----CCAU- -5' |
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9081 | 5' | -56.3 | NC_002512.2 | + | 6085 | 0.66 | 0.963878 |
Target: 5'- uCCCGGcgGUCGCCG-CCGACguccccggCGAcguccGGUc -3' miRNA: 3'- -GGGCU--UAGCGGCaGGCUGa-------GCU-----CCAu -5' |
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9081 | 5' | -56.3 | NC_002512.2 | + | 188372 | 0.66 | 0.963878 |
Target: 5'- aCCCGAGUUGCCG-CacgggaGAUggCGGGGa- -3' miRNA: 3'- -GGGCUUAGCGGCaGg-----CUGa-GCUCCau -5' |
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9081 | 5' | -56.3 | NC_002512.2 | + | 199207 | 0.66 | 0.960581 |
Target: 5'- -aCGggUUGUCGUCCGACcgCGccgcccGGUAg -3' miRNA: 3'- ggGCuuAGCGGCAGGCUGa-GCu-----CCAU- -5' |
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9081 | 5' | -56.3 | NC_002512.2 | + | 144662 | 0.66 | 0.960581 |
Target: 5'- aCCUGu-UCGCgGUCCGgGCgagCGAGGa- -3' miRNA: 3'- -GGGCuuAGCGgCAGGC-UGa--GCUCCau -5' |
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9081 | 5' | -56.3 | NC_002512.2 | + | 33313 | 0.66 | 0.960581 |
Target: 5'- -aCGGAcaCGCCGUCCGGCcaggaGAGGg- -3' miRNA: 3'- ggGCUUa-GCGGCAGGCUGag---CUCCau -5' |
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9081 | 5' | -56.3 | NC_002512.2 | + | 75103 | 0.66 | 0.960581 |
Target: 5'- gCUCGAcGUCGCCGcCUGGCggacaCGGGGa- -3' miRNA: 3'- -GGGCU-UAGCGGCaGGCUGa----GCUCCau -5' |
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9081 | 5' | -56.3 | NC_002512.2 | + | 95713 | 0.66 | 0.960581 |
Target: 5'- gCCGAAgaaCGCCGccagggUCCGGgUCGAcgGGUAc -3' miRNA: 3'- gGGCUUa--GCGGC------AGGCUgAGCU--CCAU- -5' |
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9081 | 5' | -56.3 | NC_002512.2 | + | 147517 | 0.66 | 0.960581 |
Target: 5'- uCCCGuccCGCCGaucCCGGCgCGAGGc- -3' miRNA: 3'- -GGGCuuaGCGGCa--GGCUGaGCUCCau -5' |
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9081 | 5' | -56.3 | NC_002512.2 | + | 217490 | 0.66 | 0.959553 |
Target: 5'- uCCCGGcgcCGCCG-CCGccgcccgaaggcgaGCUCGGGGg- -3' miRNA: 3'- -GGGCUua-GCGGCaGGC--------------UGAGCUCCau -5' |
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9081 | 5' | -56.3 | NC_002512.2 | + | 41600 | 0.66 | 0.957082 |
Target: 5'- aCCCG-GUUGCCGgCCGugaACUCGAccucGGUGu -3' miRNA: 3'- -GGGCuUAGCGGCaGGC---UGAGCU----CCAU- -5' |
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9081 | 5' | -56.3 | NC_002512.2 | + | 4154 | 0.66 | 0.957082 |
Target: 5'- cCCCGAcugagcgaGUCGCCG--CGGCUCGcGGg- -3' miRNA: 3'- -GGGCU--------UAGCGGCagGCUGAGCuCCau -5' |
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9081 | 5' | -56.3 | NC_002512.2 | + | 178442 | 0.66 | 0.957082 |
Target: 5'- aUCGggUCGgCGcguUCCGugUCGAucaGGUGg -3' miRNA: 3'- gGGCuuAGCgGC---AGGCugAGCU---CCAU- -5' |
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9081 | 5' | -56.3 | NC_002512.2 | + | 125032 | 0.66 | 0.954884 |
Target: 5'- gCCGAgGUCGCCGugagggacgauaccuUCCGcCUCGugaGGGUGg -3' miRNA: 3'- gGGCU-UAGCGGC---------------AGGCuGAGC---UCCAU- -5' |
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9081 | 5' | -56.3 | NC_002512.2 | + | 19912 | 0.66 | 0.953376 |
Target: 5'- cCCCGGGaaGCCGUUggCGAcCUUGAGGa- -3' miRNA: 3'- -GGGCUUagCGGCAG--GCU-GAGCUCCau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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