Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9083 | 3' | -50.4 | NC_002512.2 | + | 83469 | 0.66 | 0.998681 |
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Target: 5'- -gCGGGgcgGCGCGCAGcuUCGG-GGGGAc -3' miRNA: 3'- gaGUUUa--UGUGCGUC--AGCCaUCCCUu -5' |
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| 9083 | 3' | -50.4 | NC_002512.2 | + | 209877 | 0.67 | 0.997307 |
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Target: 5'- -cCGGcUACuACGUGGUCGGUcgcGGGGAGc -3' miRNA: 3'- gaGUUuAUG-UGCGUCAGCCA---UCCCUU- -5' |
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| 9083 | 3' | -50.4 | NC_002512.2 | + | 173019 | 0.67 | 0.996821 |
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Target: 5'- -cCAAAUGCuCGCGGUacgCGGaGGGGGGg -3' miRNA: 3'- gaGUUUAUGuGCGUCA---GCCaUCCCUU- -5' |
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| 9083 | 3' | -50.4 | NC_002512.2 | + | 193676 | 0.67 | 0.99491 |
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Target: 5'- -cCAGGaggGCuccCGCAGUCGGUcccGGGGGAc -3' miRNA: 3'- gaGUUUa--UGu--GCGUCAGCCA---UCCCUU- -5' |
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| 9083 | 3' | -50.4 | NC_002512.2 | + | 90220 | 0.69 | 0.986787 |
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Target: 5'- --gAGGUAgAUGCGGUCGGacAGGGAGa -3' miRNA: 3'- gagUUUAUgUGCGUCAGCCa-UCCCUU- -5' |
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| 9083 | 3' | -50.4 | NC_002512.2 | + | 98941 | 0.7 | 0.968113 |
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Target: 5'- -cCAGAgccGgAUGCAGUCGGgcGGGGAa -3' miRNA: 3'- gaGUUUa--UgUGCGUCAGCCauCCCUU- -5' |
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| 9083 | 3' | -50.4 | NC_002512.2 | + | 153176 | 0.71 | 0.957725 |
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Target: 5'- -aCGGGgcgGCGgGCAG-CGGUGGGGAGg -3' miRNA: 3'- gaGUUUa--UGUgCGUCaGCCAUCCCUU- -5' |
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| 9083 | 3' | -50.4 | NC_002512.2 | + | 87812 | 0.72 | 0.940582 |
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Target: 5'- gUCGAGgcggGCGgGCGGUCGG-AGGGGc -3' miRNA: 3'- gAGUUUa---UGUgCGUCAGCCaUCCCUu -5' |
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| 9083 | 3' | -50.4 | NC_002512.2 | + | 127336 | 1.07 | 0.018153 |
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Target: 5'- gCUCAAAUACACGCAGUCGGUAGGGAAa -3' miRNA: 3'- -GAGUUUAUGUGCGUCAGCCAUCCCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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