Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9143 | 3' | -50.1 | NC_002512.2 | + | 140241 | 0.66 | 0.999675 |
Target: 5'- -cGUCgaccgcUUcgUC-CGCCCCGUCGGc -3' miRNA: 3'- acCAGaa----AAuaAGaGUGGGGCAGCCc -5' |
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9143 | 3' | -50.1 | NC_002512.2 | + | 208784 | 0.66 | 0.999675 |
Target: 5'- aGGUCcgcg--UCugagacggcggUCGCCCCGgagCGGGa -3' miRNA: 3'- aCCAGaaaauaAG-----------AGUGGGGCa--GCCC- -5' |
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9143 | 3' | -50.1 | NC_002512.2 | + | 83887 | 0.66 | 0.999595 |
Target: 5'- cGGUCga------UCGCgUCGUCGGGg -3' miRNA: 3'- aCCAGaaaauaagAGUGgGGCAGCCC- -5' |
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9143 | 3' | -50.1 | NC_002512.2 | + | 153719 | 0.66 | 0.999586 |
Target: 5'- cUGGUCggg---UCUCACggcgcugUCCGUgGGGg -3' miRNA: 3'- -ACCAGaaaauaAGAGUG-------GGGCAgCCC- -5' |
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9143 | 3' | -50.1 | NC_002512.2 | + | 38998 | 0.66 | 0.999498 |
Target: 5'- cGGUUcgc----CUCGCCCCGcgacgCGGGg -3' miRNA: 3'- aCCAGaaaauaaGAGUGGGGCa----GCCC- -5' |
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9143 | 3' | -50.1 | NC_002512.2 | + | 201440 | 0.66 | 0.999465 |
Target: 5'- gGGUCgucgcgaUCUCGgccucgaggugguCCCCGUCGGc -3' miRNA: 3'- aCCAGaaaaua-AGAGU-------------GGGGCAGCCc -5' |
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9143 | 3' | -50.1 | NC_002512.2 | + | 170567 | 0.66 | 0.999381 |
Target: 5'- aGGUCgca-GUUCUC-CUCCGUCGc- -3' miRNA: 3'- aCCAGaaaaUAAGAGuGGGGCAGCcc -5' |
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9143 | 3' | -50.1 | NC_002512.2 | + | 102584 | 0.66 | 0.999381 |
Target: 5'- cUGGUCUccauccUCUCcuCCUCGUCGGc -3' miRNA: 3'- -ACCAGAaaaua-AGAGu-GGGGCAGCCc -5' |
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9143 | 3' | -50.1 | NC_002512.2 | + | 141996 | 0.67 | 0.99888 |
Target: 5'- cGGUCaacgaccgGUUgCUCGCCCUGUCGu- -3' miRNA: 3'- aCCAGaaaa----UAA-GAGUGGGGCAGCcc -5' |
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9143 | 3' | -50.1 | NC_002512.2 | + | 104411 | 0.67 | 0.998067 |
Target: 5'- uUGGUCUcgUGUUCUUcaGCgUCGUCgauGGGg -3' miRNA: 3'- -ACCAGAaaAUAAGAG--UGgGGCAG---CCC- -5' |
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9143 | 3' | -50.1 | NC_002512.2 | + | 152694 | 0.68 | 0.997705 |
Target: 5'- cGGUCUcg---UCgCGuCCCCGgUCGGGg -3' miRNA: 3'- aCCAGAaaauaAGaGU-GGGGC-AGCCC- -5' |
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9143 | 3' | -50.1 | NC_002512.2 | + | 172255 | 0.68 | 0.996261 |
Target: 5'- cGGUCUgcucgcCUCACgCCCGcUCGGu -3' miRNA: 3'- aCCAGAaaauaaGAGUG-GGGC-AGCCc -5' |
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9143 | 3' | -50.1 | NC_002512.2 | + | 131990 | 0.68 | 0.996261 |
Target: 5'- aGGUCgcgUGggC-CGCCCCGggcgcCGGGg -3' miRNA: 3'- aCCAGaaaAUaaGaGUGGGGCa----GCCC- -5' |
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9143 | 3' | -50.1 | NC_002512.2 | + | 86618 | 0.68 | 0.995639 |
Target: 5'- cGGUCg---GUUcCUCGuCCUCGUCGGc -3' miRNA: 3'- aCCAGaaaaUAA-GAGU-GGGGCAGCCc -5' |
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9143 | 3' | -50.1 | NC_002512.2 | + | 98099 | 0.69 | 0.993253 |
Target: 5'- gGGUCUUggucgccUUCcCGCCCCGcuugcCGGGg -3' miRNA: 3'- aCCAGAAaau----AAGaGUGGGGCa----GCCC- -5' |
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9143 | 3' | -50.1 | NC_002512.2 | + | 120562 | 0.69 | 0.991151 |
Target: 5'- aGGUUgaa-GUUCUgACCCCGgaugcucugCGGGa -3' miRNA: 3'- aCCAGaaaaUAAGAgUGGGGCa--------GCCC- -5' |
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9143 | 3' | -50.1 | NC_002512.2 | + | 79451 | 0.7 | 0.985422 |
Target: 5'- -uGUCUUUU-UUUUUAuUCCCGUCGGGg -3' miRNA: 3'- acCAGAAAAuAAGAGU-GGGGCAGCCC- -5' |
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9143 | 3' | -50.1 | NC_002512.2 | + | 125625 | 0.71 | 0.974719 |
Target: 5'- gGGUCaggUAUUCggUCACCCgguuggccauCGUCGGGu -3' miRNA: 3'- aCCAGaaaAUAAG--AGUGGG----------GCAGCCC- -5' |
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9143 | 3' | -50.1 | NC_002512.2 | + | 192429 | 0.74 | 0.90496 |
Target: 5'- cGGUCUc--GUUCUUcCCCCG-CGGGg -3' miRNA: 3'- aCCAGAaaaUAAGAGuGGGGCaGCCC- -5' |
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9143 | 3' | -50.1 | NC_002512.2 | + | 137710 | 0.76 | 0.8634 |
Target: 5'- cGGUUgucc--UC-CACCCCGUCGGGu -3' miRNA: 3'- aCCAGaaaauaAGaGUGGGGCAGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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