Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9148 | 5' | -49.9 | NC_002512.2 | + | 81418 | 1.05 | 0.025738 |
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Target: 5'- uUUCCAUCCUCGUUUUUCCUCCCUUGUc -3' miRNA: 3'- -AAGGUAGGAGCAAAAAGGAGGGAACA- -5' |
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| 9148 | 5' | -49.9 | NC_002512.2 | + | 171880 | 0.68 | 0.994638 |
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Target: 5'- -gCCGUCCUCGgcg-UCC-CCCUcGUc -3' miRNA: 3'- aaGGUAGGAGCaaaaAGGaGGGAaCA- -5' |
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| 9148 | 5' | -49.9 | NC_002512.2 | + | 81581 | 0.67 | 0.996647 |
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Target: 5'- gUCCAUCCgggaGUUUUUCuCUCCUa--- -3' miRNA: 3'- aAGGUAGGag--CAAAAAG-GAGGGaaca -5' |
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| 9148 | 5' | -49.9 | NC_002512.2 | + | 17274 | 0.67 | 0.997158 |
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Target: 5'- gUCCgcGUCCUCGUcg-UCCUCCUc--- -3' miRNA: 3'- aAGG--UAGGAGCAaaaAGGAGGGaaca -5' |
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| 9148 | 5' | -49.9 | NC_002512.2 | + | 106552 | 0.67 | 0.997604 |
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Target: 5'- -gUCGUCCUCGUUc-UUCUCCCgcagGUa -3' miRNA: 3'- aaGGUAGGAGCAAaaAGGAGGGaa--CA- -5' |
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| 9148 | 5' | -49.9 | NC_002512.2 | + | 104753 | 0.66 | 0.998606 |
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Target: 5'- -gCCGUCCUCGUcgccgUCgUCCCg--- -3' miRNA: 3'- aaGGUAGGAGCAaaa--AGgAGGGaaca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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