Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9166 | 3' | -46.5 | NC_002512.2 | + | 33893 | 0.77 | 0.913918 |
Target: 5'- ---cGCACGGAGAGGUGgaacgccacgucccGGUAGCAGAa -3' miRNA: 3'- agaaUGUGUCUCUCUAU--------------CCAUCGUUU- -5' |
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9166 | 3' | -46.5 | NC_002512.2 | + | 172649 | 0.71 | 0.995203 |
Target: 5'- aUCUUACGCuGGAG-GGUGGGUcGUAGAg -3' miRNA: 3'- -AGAAUGUG-UCUCuCUAUCCAuCGUUU- -5' |
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9166 | 3' | -46.5 | NC_002512.2 | + | 120549 | 0.66 | 0.999993 |
Target: 5'- cUCccuCGCAGAGA--UGGGUGGCGu- -3' miRNA: 3'- -AGaauGUGUCUCUcuAUCCAUCGUuu -5' |
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9166 | 3' | -46.5 | NC_002512.2 | + | 173026 | 0.66 | 0.999986 |
Target: 5'- cUCgcggUACGCGGAGGGggGGGU-GUAc- -3' miRNA: 3'- -AGa---AUGUGUCUCUCuaUCCAuCGUuu -5' |
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9166 | 3' | -46.5 | NC_002512.2 | + | 96614 | 0.66 | 0.99999 |
Target: 5'- ---aGCGCGGAGAcGcgGGGgagGGCGAGg -3' miRNA: 3'- agaaUGUGUCUCU-CuaUCCa--UCGUUU- -5' |
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9166 | 3' | -46.5 | NC_002512.2 | + | 107716 | 0.67 | 0.999888 |
Target: 5'- gUCgugGCGCAGgucgcAGAGGUGGGUgcgguGGCAGc -3' miRNA: 3'- -AGaa-UGUGUC-----UCUCUAUCCA-----UCGUUu -5' |
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9166 | 3' | -46.5 | NC_002512.2 | + | 57176 | 1.08 | 0.035341 |
Target: 5'- aUCUUACACAGAGAGAUAGGUAGCAAAc -3' miRNA: 3'- -AGAAUGUGUCUCUCUAUCCAUCGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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