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miRNA: 3'- -GCUGUGG---CACUuuGCG-UCUAGGAGag -5'
miRNA: 3'- gcugUGGCACUUUGCGUCUAGGagag -5'
miRNA: 3'- gCUGUGGCAcuUUGCGucuaGGAGAG- -5'
miRNA: 3'- -GCUGUGGCA-------CUUUG--CGUCuAGGAGag -5'
Results 81 - 84 of 84 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 9167 | 3' | -51.8 | NC_002512.2 | + | 133776 | 0.66 | 0.995568 |
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Target: 5'- uCGACACCcagGUGGccCGCgAGAUCUUCg- -3' miRNA: 3'- -GCUGUGG---CACUuuGCG-UCUAGGAGag -5' |
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| 9167 | 3' | -51.8 | NC_002512.2 | + | 169184 | 0.66 | 0.994859 |
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Target: 5'- ---gACCGUGAAGUGCGGGUCCa--- -3' miRNA: 3'- gcugUGGCACUUUGCGUCUAGGagag -5' |
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| 9167 | 3' | -51.8 | NC_002512.2 | + | 126410 | 0.66 | 0.994859 |
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Target: 5'- gGGCGCCGUc-GACGCcg--CCUCUCc -3' miRNA: 3'- gCUGUGGCAcuUUGCGucuaGGAGAG- -5' |
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| 9167 | 3' | -51.8 | NC_002512.2 | + | 177840 | 0.66 | 0.994783 |
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Target: 5'- gCGAUACCGUgucgaugGAGGCcaGCAGcUCCUCg- -3' miRNA: 3'- -GCUGUGGCA-------CUUUG--CGUCuAGGAGag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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