miRNA: 3'- -GCUgUGGCa-----CUUUGCgu--CU------AGGAGAG- -5'
miRNA: 3'- -GCUGugGCa--CuuUGCGuCUa--GGAGAG- -5'
miRNA: 3'- -GCugUGG-Ca---CUUUgCGUCUaGGAGag -5'
miRNA: 3'- gCUG--UGGCa-CUUUG---CGUCUAGGaGAG- -5'
Results 81 - 84 of 84 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9167 | 3' | -51.8 | NC_002512.2 | + | 219878 | 0.66 | 0.997656 |
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Target: 5'- aCGAgGCCGgggucgGAGACGagccGAccgcccUCCUCUCg -3' miRNA: 3'- -GCUgUGGCa-----CUUUGCgu--CU------AGGAGAG- -5' |
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| 9167 | 3' | -51.8 | NC_002512.2 | + | 158926 | 0.66 | 0.997656 |
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Target: 5'- aCGGCuuCGgcgGggGCGCcGGcggCCUCUCg -3' miRNA: 3'- -GCUGugGCa--CuuUGCGuCUa--GGAGAG- -5' |
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| 9167 | 3' | -51.8 | NC_002512.2 | + | 120758 | 0.66 | 0.997656 |
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Target: 5'- aCGugGCCuGgaagGAGAgGCAGAcCCUCa- -3' miRNA: 3'- -GCugUGG-Ca---CUUUgCGUCUaGGAGag -5' |
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| 9167 | 3' | -51.8 | NC_002512.2 | + | 119566 | 0.66 | 0.997656 |
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Target: 5'- gGGCggACCGcgGGAGCccgGCGGGUCCcCUCg -3' miRNA: 3'- gCUG--UGGCa-CUUUG---CGUCUAGGaGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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