miRNA: 3'- -GCUGU---------GGCaCU----UUGCGUCUAGGAGag -5'
miRNA: 3'- gCUGU-GGCACUU------------UG-CGUCUAGGa-GAG- -5'
miRNA: 3'- -GCUGuGGCa-CuuUGCG----------UCUaGGAGAG- -5'
miRNA: 3'- -GCUGUGGCACU-----------UUGCgUCUaGGAGag -5'
Results 81 - 84 of 84 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 9167 | 3' | -51.8 | NC_002512.2 | + | 220054 | 0.69 | 0.974598 |
|
Target: 5'- uCGACAuggcuccgcCCGaGAcucuGGCGCGGAUCCUCa- -3' miRNA: 3'- -GCUGU---------GGCaCU----UUGCGUCUAGGAGag -5' |
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| 9167 | 3' | -51.8 | NC_002512.2 | + | 220111 | 0.68 | 0.986951 |
|
Target: 5'- aGGCGuCCGUGAGccgccacgcgggGCgGCGGGUCCggCUCc -3' miRNA: 3'- gCUGU-GGCACUU------------UG-CGUCUAGGa-GAG- -5' |
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| 9167 | 3' | -51.8 | NC_002512.2 | + | 221838 | 0.68 | 0.988453 |
|
Target: 5'- cCGAC-CCGcgGggGCGCcucgggaccgGGA-CCUCUCg -3' miRNA: 3'- -GCUGuGGCa-CuuUGCG----------UCUaGGAGAG- -5' |
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| 9167 | 3' | -51.8 | NC_002512.2 | + | 223315 | 0.68 | 0.985304 |
|
Target: 5'- uCGACACCGUGAacggucgggagGACG-GGAcCCUCa- -3' miRNA: 3'- -GCUGUGGCACU-----------UUGCgUCUaGGAGag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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