miRNA: 3'- gCUGUGGCA---------------CU----UUGCGUCUAGGa---------GAG- -5'
miRNA: 3'- -GCUGUGGCaCUUUG------CGUCUAgGAGAg -5'
miRNA: 3'- -GCUGU---GGCACuuUG-CGuCUAGGA-GAG- -5'
miRNA: 3'- -GCUG-UgGCA--CuuUGcGUCUAGGAGAG- -5'
Results 81 - 84 of 84 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 9167 | 3' | -51.8 | NC_002512.2 | + | 103426 | 0.67 | 0.992577 |
|
Target: 5'- aGACGCCGUcgaacgcgucgucccGGuccgAGCGCGGGUCCgaggacgcggCUCg -3' miRNA: 3'- gCUGUGGCA---------------CU----UUGCGUCUAGGa---------GAG- -5' |
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| 9167 | 3' | -51.8 | NC_002512.2 | + | 208390 | 0.67 | 0.992164 |
|
Target: 5'- cCGACGCUGcGAGGCccgccuGCGGAUgCUCUa -3' miRNA: 3'- -GCUGUGGCaCUUUG------CGUCUAgGAGAg -5' |
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| 9167 | 3' | -51.8 | NC_002512.2 | + | 77049 | 0.67 | 0.992164 |
|
Target: 5'- uCGACAaucUCGUGgcGCuGCuGAUCCUgCUCg -3' miRNA: 3'- -GCUGU---GGCACuuUG-CGuCUAGGA-GAG- -5' |
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| 9167 | 3' | -51.8 | NC_002512.2 | + | 216053 | 0.67 | 0.992164 |
|
Target: 5'- gCGACgGgCGUucGggGC-CGGGUCCUCUCu -3' miRNA: 3'- -GCUG-UgGCA--CuuUGcGUCUAGGAGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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