miRNA: 3'- -GCUGUGGCA-------CUUugCGUCuAGGa--GAG- -5'
miRNA: 3'- -GCUGUGGCacuu----UGCGuCUAGGAGAg -5'
miRNA: 3'- gCUGUGGCa-CU-UUGCguCU--AGGAGag -5'
miRNA: 3'- -GCUGUGGCa--CUU-----------UGCGucuaGGAGAG- -5'
Results 81 - 84 of 84 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 9167 | 3' | -51.8 | NC_002512.2 | + | 107240 | 0.67 | 0.989686 |
|
Target: 5'- gCGGCGCCGUcagcaccGAGugGCGG-UCCgcgUUCa -3' miRNA: 3'- -GCUGUGGCA-------CUUugCGUCuAGGa--GAG- -5' |
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| 9167 | 3' | -51.8 | NC_002512.2 | + | 114528 | 0.68 | 0.988453 |
|
Target: 5'- aCGACGCCGgcgucuucACGCcGAUCUUCUg -3' miRNA: 3'- -GCUGUGGCacuu----UGCGuCUAGGAGAg -5' |
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| 9167 | 3' | -51.8 | NC_002512.2 | + | 14832 | 0.68 | 0.988453 |
|
Target: 5'- aGACGCCGccGAcGACGguGAgcUCCUCg- -3' miRNA: 3'- gCUGUGGCa-CU-UUGCguCU--AGGAGag -5' |
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| 9167 | 3' | -51.8 | NC_002512.2 | + | 121736 | 0.68 | 0.986951 |
|
Target: 5'- gCGACGCCGgagGAGucggagucuucGCGCg---CCUCUCg -3' miRNA: 3'- -GCUGUGGCa--CUU-----------UGCGucuaGGAGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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