Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9169 | 3' | -50.3 | NC_002512.2 | + | 5480 | 0.66 | 0.997295 |
Target: 5'- aUCUUCcuGCAGaGGAGAUCCCUUUCGGAa -3' miRNA: 3'- -AGAGG--UGUUaUUUCUGGGGGGAGUUU- -5' |
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9169 | 3' | -50.3 | NC_002512.2 | + | 155417 | 0.67 | 0.995602 |
Target: 5'- gCUCUGCGAccgGGAGugCCCCgUCGu- -3' miRNA: 3'- aGAGGUGUUa--UUUCugGGGGgAGUuu -5' |
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9169 | 3' | -50.3 | NC_002512.2 | + | 69650 | 0.67 | 0.994874 |
Target: 5'- cUCUCacUACAA-AAAGACCUUCCUCAu- -3' miRNA: 3'- -AGAG--GUGUUaUUUCUGGGGGGAGUuu -5' |
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9169 | 3' | -50.3 | NC_002512.2 | + | 154379 | 0.68 | 0.993127 |
Target: 5'- cUUCCGCGGcgAAAGucccGCCCCCgUCGGAg -3' miRNA: 3'- aGAGGUGUUa-UUUC----UGGGGGgAGUUU- -5' |
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9169 | 3' | -50.3 | NC_002512.2 | + | 52457 | 1.08 | 0.015715 |
Target: 5'- aUCUCCACAAUAAAGACCCCCCUCAAAg -3' miRNA: 3'- -AGAGGUGUUAUUUCUGGGGGGAGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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