Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9170 | 5' | -57.7 | NC_002512.2 | + | 171045 | 0.67 | 0.855829 |
Target: 5'- cGGGUGugAGcacGCCGCCgUCCUUCa--- -3' miRNA: 3'- -CCCACugUCu--CGGUGG-AGGAGGagac -5' |
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9170 | 5' | -57.7 | NC_002512.2 | + | 45261 | 1.11 | 0.002455 |
Target: 5'- uGGGUGACAGAGCCACCUCCUCCUCUGg -3' miRNA: 3'- -CCCACUGUCUCGGUGGAGGAGGAGAC- -5' |
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9170 | 5' | -57.7 | NC_002512.2 | + | 214635 | 0.71 | 0.657511 |
Target: 5'- cGGG-GACGcGGGCCGCCUCgcagucuCUCCUCc- -3' miRNA: 3'- -CCCaCUGU-CUCGGUGGAG-------GAGGAGac -5' |
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9170 | 5' | -57.7 | NC_002512.2 | + | 216057 | 0.71 | 0.677122 |
Target: 5'- cGGGcGuuCGGGGCCGgguccucucuccuCCUCCUCCUCUc -3' miRNA: 3'- -CCCaCu-GUCUCGGU-------------GGAGGAGGAGAc -5' |
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9170 | 5' | -57.7 | NC_002512.2 | + | 192325 | 0.71 | 0.687854 |
Target: 5'- cGGUGACGGGGCgGCUcCuCUCCUCc- -3' miRNA: 3'- cCCACUGUCUCGgUGGaG-GAGGAGac -5' |
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9170 | 5' | -57.7 | NC_002512.2 | + | 58891 | 0.7 | 0.754482 |
Target: 5'- aGGUGAaaccCGGAGCCcgagcCCUCCgUCCUCa- -3' miRNA: 3'- cCCACU----GUCUCGGu----GGAGG-AGGAGac -5' |
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9170 | 5' | -57.7 | NC_002512.2 | + | 219872 | 0.69 | 0.760922 |
Target: 5'- cGGGUGACGaGGCCggggucggagacgaGCCgaccgcCCUCCUCUc -3' miRNA: 3'- -CCCACUGUcUCGG--------------UGGa-----GGAGGAGAc -5' |
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9170 | 5' | -57.7 | NC_002512.2 | + | 96046 | 0.69 | 0.772742 |
Target: 5'- cGGUGuccucCGGAgagGCCucCCUCCUCCUCUu -3' miRNA: 3'- cCCACu----GUCU---CGGu-GGAGGAGGAGAc -5' |
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9170 | 5' | -57.7 | NC_002512.2 | + | 175617 | 0.68 | 0.824477 |
Target: 5'- gGGGUGcCAGGGUCGCCgacgCCgccgCC-CUGc -3' miRNA: 3'- -CCCACuGUCUCGGUGGa---GGa---GGaGAC- -5' |
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9170 | 5' | -57.7 | NC_002512.2 | + | 124375 | 0.68 | 0.840504 |
Target: 5'- gGGGUccaGcCAGAGCCcCCcgUCCUCC-CUGa -3' miRNA: 3'- -CCCA---CuGUCUCGGuGG--AGGAGGaGAC- -5' |
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9170 | 5' | -57.7 | NC_002512.2 | + | 3275 | 0.68 | 0.84749 |
Target: 5'- cGGUcGACgagaucaucgcccAGAGCCGCCcacgaugCCUCCUCg- -3' miRNA: 3'- cCCA-CUG-------------UCUCGGUGGa------GGAGGAGac -5' |
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9170 | 5' | -57.7 | NC_002512.2 | + | 204917 | 0.66 | 0.925234 |
Target: 5'- cGGGUGACGGGGaCGgguucgacucCCUCCuggacguUCCUCg- -3' miRNA: 3'- -CCCACUGUCUCgGU----------GGAGG-------AGGAGac -5' |
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9170 | 5' | -57.7 | NC_002512.2 | + | 219429 | 0.67 | 0.877397 |
Target: 5'- cGG-GGCGGcGGCgGCCUCCUCCg--- -3' miRNA: 3'- cCCaCUGUC-UCGgUGGAGGAGGagac -5' |
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9170 | 5' | -57.7 | NC_002512.2 | + | 105302 | 0.67 | 0.880138 |
Target: 5'- aGG-GACAGGGCgucgcucuugacgucCcggGCCUCCUCCUCg- -3' miRNA: 3'- cCCaCUGUCUCG---------------G---UGGAGGAGGAGac -5' |
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9170 | 5' | -57.7 | NC_002512.2 | + | 294 | 0.67 | 0.89077 |
Target: 5'- gGGGgccagGGCAGcccacGCCcuCCggCCUCCUCUGc -3' miRNA: 3'- -CCCa----CUGUCu----CGGu-GGa-GGAGGAGAC- -5' |
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9170 | 5' | -57.7 | NC_002512.2 | + | 229697 | 0.67 | 0.89077 |
Target: 5'- gGGGgccagGGCAGcccacGCCcuCCggCCUCCUCUGc -3' miRNA: 3'- -CCCa----CUGUCu----CGGu-GGa-GGAGGAGAC- -5' |
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9170 | 5' | -57.7 | NC_002512.2 | + | 192914 | 0.67 | 0.89077 |
Target: 5'- cGGUc-CAGAGCCGCCUCCgaugCCa--- -3' miRNA: 3'- cCCAcuGUCUCGGUGGAGGa---GGagac -5' |
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9170 | 5' | -57.7 | NC_002512.2 | + | 225299 | 0.66 | 0.897143 |
Target: 5'- cGGcccGCGGcGCCGCCUCCUCCg--- -3' miRNA: 3'- cCCac-UGUCuCGGUGGAGGAGGagac -5' |
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9170 | 5' | -57.7 | NC_002512.2 | + | 222493 | 0.66 | 0.903301 |
Target: 5'- -cGUGGCGGuGGCCGCCUUCUgCgcCUGg -3' miRNA: 3'- ccCACUGUC-UCGGUGGAGGAgGa-GAC- -5' |
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9170 | 5' | -57.7 | NC_002512.2 | + | 111811 | 0.66 | 0.903301 |
Target: 5'- cGGcGcCGGAGCCGCCcCCUCCg--- -3' miRNA: 3'- cCCaCuGUCUCGGUGGaGGAGGagac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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