Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9170 | 5' | -57.7 | NC_002512.2 | + | 228669 | 0.72 | 0.628956 |
Target: 5'- cGGGgagGACGGGGCCgggGCCgCCUuCCUCUc -3' miRNA: 3'- -CCCa--CUGUCUCGG---UGGaGGA-GGAGAc -5' |
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9170 | 5' | -57.7 | NC_002512.2 | + | 222221 | 0.72 | 0.619105 |
Target: 5'- gGGGUGGCcgucGGGGCCGCgUCCUUCgUCg- -3' miRNA: 3'- -CCCACUG----UCUCGGUGgAGGAGG-AGac -5' |
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9170 | 5' | -57.7 | NC_002512.2 | + | 207527 | 0.73 | 0.550766 |
Target: 5'- ---gGACGGGGCCGCgUCCUCCcgCUGc -3' miRNA: 3'- cccaCUGUCUCGGUGgAGGAGGa-GAC- -5' |
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9170 | 5' | -57.7 | NC_002512.2 | + | 128414 | 0.8 | 0.245379 |
Target: 5'- cGGUGGCGGGGUCGCCggagcCCUCCUCg- -3' miRNA: 3'- cCCACUGUCUCGGUGGa----GGAGGAGac -5' |
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9170 | 5' | -57.7 | NC_002512.2 | + | 45261 | 1.11 | 0.002455 |
Target: 5'- uGGGUGACAGAGCCACCUCCUCCUCUGg -3' miRNA: 3'- -CCCACUGUCUCGGUGGAGGAGGAGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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