Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9171 | 3' | -51.4 | NC_002512.2 | + | 41469 | 1.06 | 0.015325 |
Target: 5'- gACUGGCCGCUUUACAGAAAUCGGGAAc -3' miRNA: 3'- -UGACCGGCGAAAUGUCUUUAGCCCUU- -5' |
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9171 | 3' | -51.4 | NC_002512.2 | + | 52622 | 0.69 | 0.971132 |
Target: 5'- --cGGCUGUcauuCAGAAAUCGGGGu -3' miRNA: 3'- ugaCCGGCGaaauGUCUUUAGCCCUu -5' |
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9171 | 3' | -51.4 | NC_002512.2 | + | 118554 | 0.67 | 0.988339 |
Target: 5'- --cGGUCGCgu--CGGAcGUCGGGAGc -3' miRNA: 3'- ugaCCGGCGaaauGUCUuUAGCCCUU- -5' |
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9171 | 3' | -51.4 | NC_002512.2 | + | 133008 | 0.69 | 0.97394 |
Target: 5'- -aUGGCCGCcg-ACGGAGAacUGGGAGa -3' miRNA: 3'- ugACCGGCGaaaUGUCUUUa-GCCCUU- -5' |
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9171 | 3' | -51.4 | NC_002512.2 | + | 136104 | 0.66 | 0.994097 |
Target: 5'- cGCUGGCgGCggccACAGcGA-CGGGGAg -3' miRNA: 3'- -UGACCGgCGaaa-UGUCuUUaGCCCUU- -5' |
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9171 | 3' | -51.4 | NC_002512.2 | + | 176758 | 0.69 | 0.971132 |
Target: 5'- --gGGUCGCUUcggacgccUGCAGGGcUCGGGAc -3' miRNA: 3'- ugaCCGGCGAA--------AUGUCUUuAGCCCUu -5' |
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9171 | 3' | -51.4 | NC_002512.2 | + | 182300 | 0.67 | 0.986787 |
Target: 5'- cCUGGCCGU----CAGGGAcCGGGAc -3' miRNA: 3'- uGACCGGCGaaauGUCUUUaGCCCUu -5' |
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9171 | 3' | -51.4 | NC_002512.2 | + | 185002 | 0.67 | 0.991015 |
Target: 5'- cGCUGcggcccgguGCCGCUgUACAGcgccgucGUCGGGGAg -3' miRNA: 3'- -UGAC---------CGGCGAaAUGUCuu-----UAGCCCUU- -5' |
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9171 | 3' | -51.4 | NC_002512.2 | + | 190072 | 0.67 | 0.991015 |
Target: 5'- aGCUGGCCGCggccggucaGCGGGcucggcugCGGGAc -3' miRNA: 3'- -UGACCGGCGaaa------UGUCUuua-----GCCCUu -5' |
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9171 | 3' | -51.4 | NC_002512.2 | + | 205222 | 0.71 | 0.930536 |
Target: 5'- --cGGCC-CgggACGGAGAUCGGGAc -3' miRNA: 3'- ugaCCGGcGaaaUGUCUUUAGCCCUu -5' |
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9171 | 3' | -51.4 | NC_002512.2 | + | 212365 | 0.7 | 0.952577 |
Target: 5'- aGCUGGCCGCc-UACGccgacgccgacgucGAGAUCGaGGAGg -3' miRNA: 3'- -UGACCGGCGaaAUGU--------------CUUUAGC-CCUU- -5' |
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9171 | 3' | -51.4 | NC_002512.2 | + | 221250 | 0.83 | 0.342526 |
Target: 5'- gGCcGGCCGCg--GCGGAAGUCGGGGGa -3' miRNA: 3'- -UGaCCGGCGaaaUGUCUUUAGCCCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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