Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9172 | 5' | -48.1 | NC_002512.2 | + | 219301 | 0.66 | 0.999936 |
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Target: 5'- -cCCGcUUCGUCGuccgCGGCCaCGggGAGa -3' miRNA: 3'- caGGU-AAGCAGUua--GUUGG-GCuuCUU- -5' |
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| 9172 | 5' | -48.1 | NC_002512.2 | + | 106116 | 0.66 | 0.999816 |
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Target: 5'- -gCCAgaCGUCAcggGagAGCCCGAGGAGc -3' miRNA: 3'- caGGUaaGCAGU---UagUUGGGCUUCUU- -5' |
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| 9172 | 5' | -48.1 | NC_002512.2 | + | 134130 | 0.67 | 0.999764 |
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Target: 5'- gGUUC-UUCGUCcugacGAUCAACCCGGAcGGGu -3' miRNA: 3'- -CAGGuAAGCAG-----UUAGUUGGGCUU-CUU- -5' |
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| 9172 | 5' | -48.1 | NC_002512.2 | + | 180836 | 0.67 | 0.999701 |
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Target: 5'- cGUCCGc-CGUCAGUgaccucagCAGCUCGAGGAu -3' miRNA: 3'- -CAGGUaaGCAGUUA--------GUUGGGCUUCUu -5' |
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| 9172 | 5' | -48.1 | NC_002512.2 | + | 198400 | 0.67 | 0.999528 |
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Target: 5'- -aCCAUccUCGUCcuGAUCAGCCCGucuccGAGGu -3' miRNA: 3'- caGGUA--AGCAG--UUAGUUGGGC-----UUCUu -5' |
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| 9172 | 5' | -48.1 | NC_002512.2 | + | 102352 | 0.69 | 0.99808 |
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Target: 5'- gGUCCGggCGUCGGUCGuCCgGAGGu- -3' miRNA: 3'- -CAGGUaaGCAGUUAGUuGGgCUUCuu -5' |
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| 9172 | 5' | -48.1 | NC_002512.2 | + | 88984 | 0.69 | 0.996772 |
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Target: 5'- cGUCCc--CGUC-GUCGACCCGGAGc- -3' miRNA: 3'- -CAGGuaaGCAGuUAGUUGGGCUUCuu -5' |
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| 9172 | 5' | -48.1 | NC_002512.2 | + | 142798 | 0.69 | 0.996772 |
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Target: 5'- -cCCAgcaCGUCGccccgCGACCCGAGGAAc -3' miRNA: 3'- caGGUaa-GCAGUua---GUUGGGCUUCUU- -5' |
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| 9172 | 5' | -48.1 | NC_002512.2 | + | 41408 | 1.07 | 0.03101 |
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Target: 5'- gGUCCAUUCGUCAAUCAACCCGAAGAAa -3' miRNA: 3'- -CAGGUAAGCAGUUAGUUGGGCUUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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