miRNA: 3'- -CUGCUAG-GAAGAUGUG----------UGaGGCCCUc -5'
miRNA: 3'- -CUGCUAGGaagaUGuGUGa-GGCCCUC- -5'
miRNA: 3'- -CUGCUAGGAa--GAUGUGUgaGGCCCuc -5'
miRNA: 3'- -CUGCUAGGAAGAUGUGUGAGGCCCUC- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9176 | 5' | -53.1 | NC_002512.2 | + | 39139 | 0.7 | 0.922783 |
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Target: 5'- aGACGAUCgCUUCUGCGCcgacaugcggAC-CUGGGAu -3' miRNA: 3'- -CUGCUAG-GAAGAUGUG----------UGaGGCCCUc -5' |
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| 9176 | 5' | -53.1 | NC_002512.2 | + | 171846 | 0.71 | 0.892654 |
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Target: 5'- gGACGGUCCg---GC-CGCggCCGGGAGg -3' miRNA: 3'- -CUGCUAGGaagaUGuGUGa-GGCCCUC- -5' |
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| 9176 | 5' | -53.1 | NC_002512.2 | + | 90276 | 0.71 | 0.885945 |
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Target: 5'- aGAUGGUCCUgacCUGCGCGgaCCGGGc- -3' miRNA: 3'- -CUGCUAGGAa--GAUGUGUgaGGCCCuc -5' |
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| 9176 | 5' | -53.1 | NC_002512.2 | + | 35529 | 1.09 | 0.00853 |
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Target: 5'- gGACGAUCCUUCUACACACUCCGGGAGa -3' miRNA: 3'- -CUGCUAGGAAGAUGUGUGAGGCCCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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