Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9180 | 5' | -54 | NC_002512.2 | + | 91106 | 0.66 | 0.987009 |
Target: 5'- aGGACGggcccGUGGCGCUGgagcaguucgagaugUGGUCG-GACg -3' miRNA: 3'- -UCUGCa----CACUGCGGCa--------------ACCAGCgUUG- -5' |
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9180 | 5' | -54 | NC_002512.2 | + | 211774 | 0.66 | 0.986854 |
Target: 5'- aGGACGUGgaggcccggGugGCCGcgGGggccgucccgcCGCGGCg -3' miRNA: 3'- -UCUGCACa--------CugCGGCaaCCa----------GCGUUG- -5' |
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9180 | 5' | -54 | NC_002512.2 | + | 174709 | 0.66 | 0.986854 |
Target: 5'- -cGCGUG-GAUGCCGUaGGUCauggGCAcgGCg -3' miRNA: 3'- ucUGCACaCUGCGGCAaCCAG----CGU--UG- -5' |
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9180 | 5' | -54 | NC_002512.2 | + | 120192 | 0.66 | 0.986854 |
Target: 5'- uGuCG-GUGACGCCGggGGU-GCAc- -3' miRNA: 3'- uCuGCaCACUGCGGCaaCCAgCGUug -5' |
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9180 | 5' | -54 | NC_002512.2 | + | 181952 | 0.66 | 0.985221 |
Target: 5'- cGGGC-UGUGACGgUGUaUGGacCGCAACg -3' miRNA: 3'- -UCUGcACACUGCgGCA-ACCa-GCGUUG- -5' |
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9180 | 5' | -54 | NC_002512.2 | + | 219868 | 0.66 | 0.981497 |
Target: 5'- cGGACGgGUGACgagGCCGg-GGUCGgaGACg -3' miRNA: 3'- -UCUGCaCACUG---CGGCaaCCAGCg-UUG- -5' |
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9180 | 5' | -54 | NC_002512.2 | + | 133880 | 0.66 | 0.97939 |
Target: 5'- gGGGCGUuc--CGCCuGgcGGUCGCGGCg -3' miRNA: 3'- -UCUGCAcacuGCGG-CaaCCAGCGUUG- -5' |
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9180 | 5' | -54 | NC_002512.2 | + | 200752 | 0.66 | 0.97939 |
Target: 5'- cGGGCGUG-GuCGUCGUacauggUGGUCGCc-- -3' miRNA: 3'- -UCUGCACaCuGCGGCA------ACCAGCGuug -5' |
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9180 | 5' | -54 | NC_002512.2 | + | 158933 | 0.67 | 0.97465 |
Target: 5'- cGGCGgg-GGCGCCGgcggccucucGGUCaGCAACg -3' miRNA: 3'- uCUGCacaCUGCGGCaa--------CCAG-CGUUG- -5' |
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9180 | 5' | -54 | NC_002512.2 | + | 204067 | 0.67 | 0.969162 |
Target: 5'- cGACGUG-GACGUCGUcGG-CGgGAUg -3' miRNA: 3'- uCUGCACaCUGCGGCAaCCaGCgUUG- -5' |
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9180 | 5' | -54 | NC_002512.2 | + | 128821 | 0.67 | 0.969162 |
Target: 5'- cGGCG-GcGGCGCUGUcGG-CGCAGCu -3' miRNA: 3'- uCUGCaCaCUGCGGCAaCCaGCGUUG- -5' |
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9180 | 5' | -54 | NC_002512.2 | + | 226780 | 0.67 | 0.969162 |
Target: 5'- aGGACGUcucGggcGACGCCG-UGGUCuCGGCc -3' miRNA: 3'- -UCUGCA---Ca--CUGCGGCaACCAGcGUUG- -5' |
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9180 | 5' | -54 | NC_002512.2 | + | 107331 | 0.67 | 0.962875 |
Target: 5'- uGACG-GUGGCGUCGUacuUGGUgaCGCGcGCg -3' miRNA: 3'- uCUGCaCACUGCGGCA---ACCA--GCGU-UG- -5' |
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9180 | 5' | -54 | NC_002512.2 | + | 157115 | 0.68 | 0.959419 |
Target: 5'- cGACGUcccucccGGCGUCGggGGUCgGCGGCg -3' miRNA: 3'- uCUGCAca-----CUGCGGCaaCCAG-CGUUG- -5' |
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9180 | 5' | -54 | NC_002512.2 | + | 115360 | 0.68 | 0.959419 |
Target: 5'- cGGCGUGaaGACGCCGgc-GUCGUAcgGCa -3' miRNA: 3'- uCUGCACa-CUGCGGCaacCAGCGU--UG- -5' |
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9180 | 5' | -54 | NC_002512.2 | + | 219029 | 0.69 | 0.934038 |
Target: 5'- cGACGUGcUGgucuACGUCGUcGGcCGCGGCg -3' miRNA: 3'- uCUGCAC-AC----UGCGGCAaCCaGCGUUG- -5' |
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9180 | 5' | -54 | NC_002512.2 | + | 96814 | 0.69 | 0.923748 |
Target: 5'- aAGACGUG-GACGCUGUcgauccuaUGcugCGCGGCg -3' miRNA: 3'- -UCUGCACaCUGCGGCA--------ACca-GCGUUG- -5' |
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9180 | 5' | -54 | NC_002512.2 | + | 202156 | 0.7 | 0.893988 |
Target: 5'- gGGGCGg--GugGUCGUucccgUGGUCGUGACg -3' miRNA: 3'- -UCUGCacaCugCGGCA-----ACCAGCGUUG- -5' |
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9180 | 5' | -54 | NC_002512.2 | + | 116115 | 0.7 | 0.880511 |
Target: 5'- aGGACGagugcaaucgcUGUGcACGCCGcguaucgUGGUCgGCAACa -3' miRNA: 3'- -UCUGC-----------ACAC-UGCGGCa------ACCAG-CGUUG- -5' |
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9180 | 5' | -54 | NC_002512.2 | + | 161685 | 0.71 | 0.851027 |
Target: 5'- uGGACGUGUGGCGCgUGgaagaUGGagaCGCGGCc -3' miRNA: 3'- -UCUGCACACUGCG-GCa----ACCa--GCGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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