Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9183 | 5' | -51.6 | NC_002512.2 | + | 175161 | 0.66 | 0.997589 |
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Target: 5'- aGGCCUUCaGGUC-CGAGAuGUCGUa-- -3' miRNA: 3'- gCUGGAAG-CUAGcGCUCU-CAGUAguu -5' |
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| 9183 | 5' | -51.6 | NC_002512.2 | + | 128050 | 0.67 | 0.994708 |
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Target: 5'- aCGGCCccgaCGGUCGCG-GcGUCGUCGu -3' miRNA: 3'- -GCUGGaa--GCUAGCGCuCuCAGUAGUu -5' |
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| 9183 | 5' | -51.6 | NC_002512.2 | + | 155461 | 0.67 | 0.992964 |
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Target: 5'- uCGACCUgaccgUCGAcCGCGAGu-UCGUCGg -3' miRNA: 3'- -GCUGGA-----AGCUaGCGCUCucAGUAGUu -5' |
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| 9183 | 5' | -51.6 | NC_002512.2 | + | 195053 | 0.71 | 0.936492 |
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Target: 5'- gCGGCCcgCGggCGgGAGGGUCGUCc- -3' miRNA: 3'- -GCUGGaaGCuaGCgCUCUCAGUAGuu -5' |
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| 9183 | 5' | -51.6 | NC_002512.2 | + | 146825 | 0.74 | 0.852954 |
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Target: 5'- aGACCUUCGcgCGCGAG-GUCGc--- -3' miRNA: 3'- gCUGGAAGCuaGCGCUCuCAGUaguu -5' |
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| 9183 | 5' | -51.6 | NC_002512.2 | + | 3525 | 1.06 | 0.01645 |
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Target: 5'- cCGACCUUCGAUCGCGAGAGUCAUCAAc -3' miRNA: 3'- -GCUGGAAGCUAGCGCUCUCAGUAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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