Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9185 | 5' | -54.3 | NC_002512.2 | + | 3541 | 1.08 | 0.008028 |
Target: 5'- cCGAAGCCCGGGUAGAAGAUCCGUCUGc -3' miRNA: 3'- -GCUUCGGGCCCAUCUUCUAGGCAGAC- -5' |
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9185 | 5' | -54.3 | NC_002512.2 | + | 2774 | 0.8 | 0.384364 |
Target: 5'- gGAGGCCCGGGgccgccggcgucggGGGAGAUCCGUUc- -3' miRNA: 3'- gCUUCGGGCCCa-------------UCUUCUAGGCAGac -5' |
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9185 | 5' | -54.3 | NC_002512.2 | + | 221254 | 0.8 | 0.405899 |
Target: 5'- aCGAGGCUCGGGUAGAucgaggacGGUCCGUUUa -3' miRNA: 3'- -GCUUCGGGCCCAUCUu-------CUAGGCAGAc -5' |
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9185 | 5' | -54.3 | NC_002512.2 | + | 18358 | 0.79 | 0.45837 |
Target: 5'- gGAGGCCCGGGUGGccgcGGGggCCGUCc- -3' miRNA: 3'- gCUUCGGGCCCAUC----UUCuaGGCAGac -5' |
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9185 | 5' | -54.3 | NC_002512.2 | + | 32724 | 0.75 | 0.65262 |
Target: 5'- aGAGGCgCGGGUGGAAGAcggCCGUg-- -3' miRNA: 3'- gCUUCGgGCCCAUCUUCUa--GGCAgac -5' |
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9185 | 5' | -54.3 | NC_002512.2 | + | 28202 | 0.71 | 0.861726 |
Target: 5'- gCGAcgGGCCCGGGUacguGGGAGA-CCGcUCUc -3' miRNA: 3'- -GCU--UCGGGCCCA----UCUUCUaGGC-AGAc -5' |
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9185 | 5' | -54.3 | NC_002512.2 | + | 32808 | 0.7 | 0.894473 |
Target: 5'- gCGgcGCCCGGcGgcuggAGAAGAUCCcccacugcccgcacGUCUGc -3' miRNA: 3'- -GCuuCGGGCC-Ca----UCUUCUAGG--------------CAGAC- -5' |
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9185 | 5' | -54.3 | NC_002512.2 | + | 33515 | 0.7 | 0.896402 |
Target: 5'- cCGGuucGCCCGGGUGGccGAcgCCGUgUGu -3' miRNA: 3'- -GCUu--CGGGCCCAUCuuCUa-GGCAgAC- -5' |
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9185 | 5' | -54.3 | NC_002512.2 | + | 76660 | 0.7 | 0.90269 |
Target: 5'- gGGAGCCCGGGacGGggGAaCCGgggCg- -3' miRNA: 3'- gCUUCGGGCCCa-UCuuCUaGGCa--Gac -5' |
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9185 | 5' | -54.3 | NC_002512.2 | + | 135192 | 0.69 | 0.908754 |
Target: 5'- cCGGAGCCCccGGaGGAAGAUCCGa--- -3' miRNA: 3'- -GCUUCGGGc-CCaUCUUCUAGGCagac -5' |
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9185 | 5' | -54.3 | NC_002512.2 | + | 25420 | 0.69 | 0.914593 |
Target: 5'- aCGGcgGGCCCGGGgcGggGggCCGg--- -3' miRNA: 3'- -GCU--UCGGGCCCauCuuCuaGGCagac -5' |
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9185 | 5' | -54.3 | NC_002512.2 | + | 29465 | 0.69 | 0.914593 |
Target: 5'- cCGgcGUUCGGGUAGAAGGUguacucggUCGUCUc -3' miRNA: 3'- -GCuuCGGGCCCAUCUUCUA--------GGCAGAc -5' |
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9185 | 5' | -54.3 | NC_002512.2 | + | 121705 | 0.69 | 0.920203 |
Target: 5'- cCGggGCCgGGGgccGGggGGUCCcUCc- -3' miRNA: 3'- -GCuuCGGgCCCa--UCuuCUAGGcAGac -5' |
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9185 | 5' | -54.3 | NC_002512.2 | + | 44832 | 0.69 | 0.930737 |
Target: 5'- uCGAGGCCCGGGUcGGcguccaGGAUCCu---- -3' miRNA: 3'- -GCUUCGGGCCCAuCU------UCUAGGcagac -5' |
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9185 | 5' | -54.3 | NC_002512.2 | + | 42607 | 0.68 | 0.944824 |
Target: 5'- cCGAAGCCCGGGggcacuucgAGgcGAcgUCCGcCa- -3' miRNA: 3'- -GCUUCGGGCCCa--------UCuuCU--AGGCaGac -5' |
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9185 | 5' | -54.3 | NC_002512.2 | + | 3766 | 0.67 | 0.960473 |
Target: 5'- cCGcGGGCUCGGGUGGAGGG-CCGa--- -3' miRNA: 3'- -GC-UUCGGGCCCAUCUUCUaGGCagac -5' |
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9185 | 5' | -54.3 | NC_002512.2 | + | 10261 | 0.67 | 0.962857 |
Target: 5'- aCGAGGCCgGGGUcGGAGAcgagccgaccgcccUCCucucgucucgucGUCUGg -3' miRNA: 3'- -GCUUCGGgCCCAuCUUCU--------------AGG------------CAGAC- -5' |
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9185 | 5' | -54.3 | NC_002512.2 | + | 3268 | 0.67 | 0.963847 |
Target: 5'- gGAGGCCCGGGaggccaGGAAGGcCCGg--- -3' miRNA: 3'- gCUUCGGGCCCa-----UCUUCUaGGCagac -5' |
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9185 | 5' | -54.3 | NC_002512.2 | + | 229939 | 0.67 | 0.969981 |
Target: 5'- aGggGCCCGGGcggAGAGGGagCGg--- -3' miRNA: 3'- gCuuCGGGCCCa--UCUUCUagGCagac -5' |
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9185 | 5' | -54.3 | NC_002512.2 | + | 536 | 0.67 | 0.969981 |
Target: 5'- aGggGCCCGGGcggAGAGGGagCGg--- -3' miRNA: 3'- gCuuCGGGCCCa--UCUUCUagGCagac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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