Results 21 - 32 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9185 | 5' | -54.3 | NC_002512.2 | + | 44832 | 0.69 | 0.930737 |
Target: 5'- uCGAGGCCCGGGUcGGcguccaGGAUCCu---- -3' miRNA: 3'- -GCUUCGGGCCCAuCU------UCUAGGcagac -5' |
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9185 | 5' | -54.3 | NC_002512.2 | + | 63111 | 0.66 | 0.983893 |
Target: 5'- ---cGUCCGGGacgGGAGGAgagCCGUCc- -3' miRNA: 3'- gcuuCGGGCCCa--UCUUCUa--GGCAGac -5' |
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9185 | 5' | -54.3 | NC_002512.2 | + | 70744 | 0.67 | 0.972751 |
Target: 5'- gCGgcGUCgGGGcGGAAGGUCCgGUCg- -3' miRNA: 3'- -GCuuCGGgCCCaUCUUCUAGG-CAGac -5' |
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9185 | 5' | -54.3 | NC_002512.2 | + | 76660 | 0.7 | 0.90269 |
Target: 5'- gGGAGCCCGGGacGGggGAaCCGgggCg- -3' miRNA: 3'- gCUUCGGGCCCa-UCuuCUaGGCa--Gac -5' |
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9185 | 5' | -54.3 | NC_002512.2 | + | 83331 | 0.66 | 0.982003 |
Target: 5'- gGAGGCCCGGGaccuGGAGA-CCuUCg- -3' miRNA: 3'- gCUUCGGGCCCau--CUUCUaGGcAGac -5' |
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9185 | 5' | -54.3 | NC_002512.2 | + | 103746 | 0.66 | 0.982003 |
Target: 5'- aGAGcGCCCGGGgccccGGGcgCCGUCg- -3' miRNA: 3'- gCUU-CGGGCCCauc--UUCuaGGCAGac -5' |
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9185 | 5' | -54.3 | NC_002512.2 | + | 121705 | 0.69 | 0.920203 |
Target: 5'- cCGggGCCgGGGgccGGggGGUCCcUCc- -3' miRNA: 3'- -GCuuCGGgCCCa--UCuuCUAGGcAGac -5' |
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9185 | 5' | -54.3 | NC_002512.2 | + | 123665 | 0.66 | 0.983893 |
Target: 5'- gCGgcGgCCGGGcucggGGAAGcgGUCCGUCg- -3' miRNA: 3'- -GCuuCgGGCCCa----UCUUC--UAGGCAGac -5' |
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9185 | 5' | -54.3 | NC_002512.2 | + | 131882 | 0.67 | 0.969981 |
Target: 5'- gGGAGCCggaGGGggagagGGggGGUCgGUCg- -3' miRNA: 3'- gCUUCGGg--CCCa-----UCuuCUAGgCAGac -5' |
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9185 | 5' | -54.3 | NC_002512.2 | + | 135192 | 0.69 | 0.908754 |
Target: 5'- cCGGAGCCCccGGaGGAAGAUCCGa--- -3' miRNA: 3'- -GCUUCGGGc-CCaUCUUCUAGGCagac -5' |
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9185 | 5' | -54.3 | NC_002512.2 | + | 221254 | 0.8 | 0.405899 |
Target: 5'- aCGAGGCUCGGGUAGAucgaggacGGUCCGUUUa -3' miRNA: 3'- -GCUUCGGGCCCAUCUu-------CUAGGCAGAc -5' |
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9185 | 5' | -54.3 | NC_002512.2 | + | 229939 | 0.67 | 0.969981 |
Target: 5'- aGggGCCCGGGcggAGAGGGagCGg--- -3' miRNA: 3'- gCuuCGGGCCCa--UCUUCUagGCagac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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