Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 92 | 5' | -55 | AC_000006.1 | + | 8895 | 0.66 | 0.578427 |
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Target: 5'- uAGGUgCCUCAACcgaGCGAGCUCAg--- -3' miRNA: 3'- cUCCG-GGAGUUGa--UGCUCGAGUacca -5' |
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| 92 | 5' | -55 | AC_000006.1 | + | 23625 | 0.66 | 0.567027 |
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Target: 5'- cAGGCCCUggagCAGCgGCGcaAGCUCAUGc- -3' miRNA: 3'- cUCCGGGA----GUUGaUGC--UCGAGUACca -5' |
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| 92 | 5' | -55 | AC_000006.1 | + | 30409 | 0.67 | 0.489316 |
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Target: 5'- cAGGCaaagaUCAcCUACGAGCUCGUGu- -3' miRNA: 3'- cUCCGgg---AGUuGAUGCUCGAGUACca -5' |
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| 92 | 5' | -55 | AC_000006.1 | + | 26044 | 0.67 | 0.478604 |
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Target: 5'- cAGGCuCCUgGAagACGAGCUCAcGGUc -3' miRNA: 3'- cUCCG-GGAgUUgaUGCUCGAGUaCCA- -5' |
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| 92 | 5' | -55 | AC_000006.1 | + | 28153 | 0.73 | 0.218231 |
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Target: 5'- --cGCCCUCAACcGCGAGCU-GUGGg -3' miRNA: 3'- cucCGGGAGUUGaUGCUCGAgUACCa -5' |
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| 92 | 5' | -55 | AC_000006.1 | + | 26786 | 0.79 | 0.086249 |
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Target: 5'- -uGGCCCcgcgCAGCUACGGGCUCAUGc- -3' miRNA: 3'- cuCCGGGa---GUUGAUGCUCGAGUACca -5' |
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| 92 | 5' | -55 | AC_000006.1 | + | 29799 | 1.07 | 0.000696 |
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Target: 5'- cGAGGCCCUCAACUACGAGCUCAUGGUc -3' miRNA: 3'- -CUCCGGGAGUUGAUGCUCGAGUACCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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