Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9235 | 5' | -60.2 | NC_002512.2 | + | 42773 | 1.08 | 0.002234 |
Target: 5'- cCCUGCCCCUGGUCCACGUAGGGCUGUc -3' miRNA: 3'- -GGACGGGGACCAGGUGCAUCCCGACA- -5' |
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9235 | 5' | -60.2 | NC_002512.2 | + | 107636 | 0.78 | 0.220889 |
Target: 5'- gCUGCCCgUGGUUCGCGUGGuGGCUc- -3' miRNA: 3'- gGACGGGgACCAGGUGCAUC-CCGAca -5' |
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9235 | 5' | -60.2 | NC_002512.2 | + | 98754 | 0.74 | 0.367119 |
Target: 5'- cCCUGCCCCgccaucgGGUCCugGccgucggGGGGCa-- -3' miRNA: 3'- -GGACGGGGa------CCAGGugCa------UCCCGaca -5' |
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9235 | 5' | -60.2 | NC_002512.2 | + | 9436 | 0.71 | 0.558095 |
Target: 5'- aCUUGCCCgUGGagcCCACGUAGagcuccggcGGCUGg -3' miRNA: 3'- -GGACGGGgACCa--GGUGCAUC---------CCGACa -5' |
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9235 | 5' | -60.2 | NC_002512.2 | + | 95651 | 0.69 | 0.664166 |
Target: 5'- gUCUGCCUgaGGaggaaCGcCGUGGGGCUGUa -3' miRNA: 3'- -GGACGGGgaCCag---GU-GCAUCCCGACA- -5' |
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9235 | 5' | -60.2 | NC_002512.2 | + | 129754 | 0.67 | 0.767269 |
Target: 5'- aCgUGCCuCCUGGUCCGCcUAGaGGacCUGg -3' miRNA: 3'- -GgACGG-GGACCAGGUGcAUC-CC--GACa -5' |
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9235 | 5' | -60.2 | NC_002512.2 | + | 67489 | 0.67 | 0.767269 |
Target: 5'- cCCU-CCUC-GGUCgGCGUcuuGGGGCUGg -3' miRNA: 3'- -GGAcGGGGaCCAGgUGCA---UCCCGACa -5' |
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9235 | 5' | -60.2 | NC_002512.2 | + | 2677 | 0.67 | 0.776151 |
Target: 5'- uCCgccGCCuCCUGGUCCGCGcgcGGGaGCg-- -3' miRNA: 3'- -GGa--CGG-GGACCAGGUGCa--UCC-CGaca -5' |
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9235 | 5' | -60.2 | NC_002512.2 | + | 103264 | 0.66 | 0.826758 |
Target: 5'- gCUgGUCCUUGGUguagaCCACGUAGGacccGCUGa -3' miRNA: 3'- gGA-CGGGGACCA-----GGUGCAUCC----CGACa -5' |
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9235 | 5' | -60.2 | NC_002512.2 | + | 185480 | 0.66 | 0.81045 |
Target: 5'- uCCUGCCgCCgcaGGUCauggcgCACGccGGGCUGc -3' miRNA: 3'- -GGACGG-GGa--CCAG------GUGCauCCCGACa -5' |
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9235 | 5' | -60.2 | NC_002512.2 | + | 197705 | 0.66 | 0.802075 |
Target: 5'- gCCUGCCCaUGGcCCGCGaccccAGGGUgcgGg -3' miRNA: 3'- -GGACGGGgACCaGGUGCa----UCCCGa--Ca -5' |
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9235 | 5' | -60.2 | NC_002512.2 | + | 135661 | 0.67 | 0.784918 |
Target: 5'- cCCgugGUgaUCCUGGUCguccuCGUGGGGCUGc -3' miRNA: 3'- -GGa--CG--GGGACCAGgu---GCAUCCCGACa -5' |
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9235 | 5' | -60.2 | NC_002512.2 | + | 92443 | 0.68 | 0.74369 |
Target: 5'- uCCUGUCCCugagcgaacUGGcCCACGUGGuguacgagacgagccGGCUGc -3' miRNA: 3'- -GGACGGGG---------ACCaGGUGCAUC---------------CCGACa -5' |
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9235 | 5' | -60.2 | NC_002512.2 | + | 187138 | 0.69 | 0.673806 |
Target: 5'- gCUGCCCCUGuUCCugGggucGGGgaGg -3' miRNA: 3'- gGACGGGGACcAGGugCau--CCCgaCa -5' |
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9235 | 5' | -60.2 | NC_002512.2 | + | 93257 | 0.69 | 0.635137 |
Target: 5'- gCUGCCCCUGuccaaggCCACGcuggAGGGCg-- -3' miRNA: 3'- gGACGGGGACca-----GGUGCa---UCCCGaca -5' |
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9235 | 5' | -60.2 | NC_002512.2 | + | 144512 | 0.73 | 0.431518 |
Target: 5'- gCUGCCCggaCUcGGUCCGCGUGGGcGCgGUc -3' miRNA: 3'- gGACGGG---GA-CCAGGUGCAUCC-CGaCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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