Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 9292 | 5' | -58.3 | NC_002512.2 | + | 8918 | 0.73 | 0.552557 |
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Target: 5'- uGGCCAGGGCGgCCUcgGCcGGGUCGGc -3' miRNA: 3'- -CUGGUCUCGUgGGAcaCGcUCCAGCC- -5' |
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| 9292 | 5' | -58.3 | NC_002512.2 | + | 201066 | 0.74 | 0.478038 |
|
Target: 5'- cGACCGG-GC-CCacaUGUGCGGGGUCuGGg -3' miRNA: 3'- -CUGGUCuCGuGGg--ACACGCUCCAG-CC- -5' |
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| 9292 | 5' | -58.3 | NC_002512.2 | + | 74572 | 0.74 | 0.478038 |
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Target: 5'- cGACCuGGGCGCUCUgGUGCucGAGGcCGGg -3' miRNA: 3'- -CUGGuCUCGUGGGA-CACG--CUCCaGCC- -5' |
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| 9292 | 5' | -58.3 | NC_002512.2 | + | 194638 | 0.75 | 0.45137 |
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Target: 5'- uGACaCGGGGCACCC-GUcCGAGGUCGa -3' miRNA: 3'- -CUG-GUCUCGUGGGaCAcGCUCCAGCc -5' |
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| 9292 | 5' | -58.3 | NC_002512.2 | + | 44346 | 0.76 | 0.376625 |
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Target: 5'- cGACCauucgcccuguGGuGCGCCCUGUGUGuGGGUUGGa -3' miRNA: 3'- -CUGG-----------UCuCGUGGGACACGC-UCCAGCC- -5' |
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| 9292 | 5' | -58.3 | NC_002512.2 | + | 86036 | 1.09 | 0.002793 |
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Target: 5'- cGACCAGAGCACCCUGUGCGAGGUCGGc -3' miRNA: 3'- -CUGGUCUCGUGGGACACGCUCCAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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