Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 9292 | 5' | -58.3 | NC_002512.2 | + | 187123 | 0.71 | 0.630185 |
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Target: 5'- gGACCAgGGGCAggggcugcCCCUGUuccUGGGGUCGGg -3' miRNA: 3'- -CUGGU-CUCGU--------GGGACAc--GCUCCAGCC- -5' |
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| 9292 | 5' | -58.3 | NC_002512.2 | + | 194638 | 0.75 | 0.45137 |
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Target: 5'- uGACaCGGGGCACCC-GUcCGAGGUCGa -3' miRNA: 3'- -CUG-GUCUCGUGGGaCAcGCUCCAGCc -5' |
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| 9292 | 5' | -58.3 | NC_002512.2 | + | 201066 | 0.74 | 0.478038 |
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Target: 5'- cGACCGG-GC-CCacaUGUGCGGGGUCuGGg -3' miRNA: 3'- -CUGGUCuCGuGGg--ACACGCUCCAG-CC- -5' |
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| 9292 | 5' | -58.3 | NC_002512.2 | + | 201683 | 0.67 | 0.862671 |
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Target: 5'- cGGCgAGGGCGgCCgcgucugGUGCGGGaUCGGc -3' miRNA: 3'- -CUGgUCUCGUgGGa------CACGCUCcAGCC- -5' |
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| 9292 | 5' | -58.3 | NC_002512.2 | + | 211903 | 0.66 | 0.902572 |
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Target: 5'- -uCCGGGGCugCCgcgucGU-CGAGcGUCGGg -3' miRNA: 3'- cuGGUCUCGugGGa----CAcGCUC-CAGCC- -5' |
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| 9292 | 5' | -58.3 | NC_002512.2 | + | 219216 | 0.7 | 0.698254 |
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Target: 5'- uGCCGG-GCcgGCCUcG-GCGAGGUCGGg -3' miRNA: 3'- cUGGUCuCG--UGGGaCaCGCUCCAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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