Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 9292 | 5' | -58.3 | NC_002512.2 | + | 38929 | 0.67 | 0.854586 |
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Target: 5'- gGGCCGccgcggcGGGCGCCCgcGUGCGcccGGGUCuGGu -3' miRNA: 3'- -CUGGU-------CUCGUGGGa-CACGC---UCCAG-CC- -5' |
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| 9292 | 5' | -58.3 | NC_002512.2 | + | 32567 | 0.68 | 0.807625 |
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Target: 5'- cGCCGG-GCGCCgCggGUGcCGGGGUCGu -3' miRNA: 3'- cUGGUCuCGUGG-Ga-CAC-GCUCCAGCc -5' |
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| 9292 | 5' | -58.3 | NC_002512.2 | + | 8918 | 0.73 | 0.552557 |
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Target: 5'- uGGCCAGGGCGgCCUcgGCcGGGUCGGc -3' miRNA: 3'- -CUGGUCUCGUgGGAcaCGcUCCAGCC- -5' |
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| 9292 | 5' | -58.3 | NC_002512.2 | + | 7422 | 0.68 | 0.790488 |
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Target: 5'- cGACCGcGAGgGCCg-GcGCGAGGUUGGc -3' miRNA: 3'- -CUGGU-CUCgUGGgaCaCGCUCCAGCC- -5' |
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| 9292 | 5' | -58.3 | NC_002512.2 | + | 4293 | 0.69 | 0.733396 |
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Target: 5'- cGGCCAGcuggucgaagacccGGCGCCCcu--CGAGGUCGGc -3' miRNA: 3'- -CUGGUC--------------UCGUGGGacacGCUCCAGCC- -5' |
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| 9292 | 5' | -58.3 | NC_002512.2 | + | 3795 | 0.68 | 0.832224 |
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Target: 5'- gGACCGGAGCAguCCCgagaGCGAGccGUCGa -3' miRNA: 3'- -CUGGUCUCGU--GGGaca-CGCUC--CAGCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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