Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 9292 | 5' | -58.3 | NC_002512.2 | + | 187123 | 0.71 | 0.630185 |
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Target: 5'- gGACCAgGGGCAggggcugcCCCUGUuccUGGGGUCGGg -3' miRNA: 3'- -CUGGU-CUCGU--------GGGACAc--GCUCCAGCC- -5' |
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| 9292 | 5' | -58.3 | NC_002512.2 | + | 163052 | 0.69 | 0.772831 |
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Target: 5'- aACaAGAGCgaaGCCCUGUGCGAGuGgaaaCGGu -3' miRNA: 3'- cUGgUCUCG---UGGGACACGCUC-Ca---GCC- -5' |
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| 9292 | 5' | -58.3 | NC_002512.2 | + | 156819 | 0.68 | 0.790488 |
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Target: 5'- -cUCGGucGGCAaCCUGUGCGAGGccugCGGg -3' miRNA: 3'- cuGGUC--UCGUgGGACACGCUCCa---GCC- -5' |
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| 9292 | 5' | -58.3 | NC_002512.2 | + | 124258 | 0.68 | 0.790488 |
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Target: 5'- cGACaCGGuGCACCCgacgGCGGcGUCGGa -3' miRNA: 3'- -CUG-GUCuCGUGGGaca-CGCUcCAGCC- -5' |
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| 9292 | 5' | -58.3 | NC_002512.2 | + | 185420 | 0.68 | 0.799126 |
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Target: 5'- -cCCGGGGCGCCCUGgaggucuuCGAGGcCGu -3' miRNA: 3'- cuGGUCUCGUGGGACac------GCUCCaGCc -5' |
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| 9292 | 5' | -58.3 | NC_002512.2 | + | 131214 | 0.66 | 0.914204 |
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Target: 5'- cGGCCAG-GCGCUCgacGU-CGcGGUCGGa -3' miRNA: 3'- -CUGGUCuCGUGGGa--CAcGCuCCAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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