Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9323 | 3' | -54.7 | NC_002512.2 | + | 214381 | 0.65 | 0.982114 |
Target: 5'- cGGGAccacaucguCGUCUUCGgccacuucgggccGGAGCGcgccgCGCCCGa -3' miRNA: 3'- uCCCU---------GCAGAAGU-------------CCUUGCa----GUGGGU- -5' |
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9323 | 3' | -54.7 | NC_002512.2 | + | 118540 | 0.65 | 0.981719 |
Target: 5'- gGGGGaaacgaacgcggucGCGUCggacgUCGGGAGCGUCgaaagggaGCCgCGa -3' miRNA: 3'- -UCCC--------------UGCAGa----AGUCCUUGCAG--------UGG-GU- -5' |
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9323 | 3' | -54.7 | NC_002512.2 | + | 92667 | 0.66 | 0.978092 |
Target: 5'- cGGGACGgccgcccgCgcCGGGcGCGcCGCCCAc -3' miRNA: 3'- uCCCUGCa-------GaaGUCCuUGCaGUGGGU- -5' |
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9323 | 3' | -54.7 | NC_002512.2 | + | 221600 | 0.66 | 0.976453 |
Target: 5'- gAGGGGgGUCgguggacggcgaggaGGGAGCGggucCACCCGg -3' miRNA: 3'- -UCCCUgCAGaag------------UCCUUGCa---GUGGGU- -5' |
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9323 | 3' | -54.7 | NC_002512.2 | + | 204070 | 0.66 | 0.975724 |
Target: 5'- cGuGGACGUCgucggCGGGAugGUCuACgCGg -3' miRNA: 3'- uC-CCUGCAGaa---GUCCUugCAG-UGgGU- -5' |
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9323 | 3' | -54.7 | NC_002512.2 | + | 209075 | 0.66 | 0.974979 |
Target: 5'- --cGACGUCUUCgugcGGGAGCccgacguccgggucGUCACCCc -3' miRNA: 3'- uccCUGCAGAAG----UCCUUG--------------CAGUGGGu -5' |
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9323 | 3' | -54.7 | NC_002512.2 | + | 177555 | 0.66 | 0.973175 |
Target: 5'- -aGGAuCGUCUgguUCGGGGcCGUCGCCa- -3' miRNA: 3'- ucCCU-GCAGA---AGUCCUuGCAGUGGgu -5' |
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9323 | 3' | -54.7 | NC_002512.2 | + | 216252 | 0.67 | 0.953724 |
Target: 5'- -cGGuCGUCaUCAGGuGCGcCGCCCGc -3' miRNA: 3'- ucCCuGCAGaAGUCCuUGCaGUGGGU- -5' |
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9323 | 3' | -54.7 | NC_002512.2 | + | 178243 | 0.68 | 0.945539 |
Target: 5'- cGGGGACGgcgcggUCUcCGGGucCGUCGCCa- -3' miRNA: 3'- -UCCCUGC------AGAaGUCCuuGCAGUGGgu -5' |
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9323 | 3' | -54.7 | NC_002512.2 | + | 187158 | 0.68 | 0.941112 |
Target: 5'- cGGGGAgGUCcaUGGuGGACGUgGCCCGc -3' miRNA: 3'- -UCCCUgCAGaaGUC-CUUGCAgUGGGU- -5' |
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9323 | 3' | -54.7 | NC_002512.2 | + | 183958 | 0.68 | 0.941112 |
Target: 5'- cGGGACG-CgcgcUCGGGcguCGUCGCCUg -3' miRNA: 3'- uCCCUGCaGa---AGUCCuu-GCAGUGGGu -5' |
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9323 | 3' | -54.7 | NC_002512.2 | + | 166619 | 0.68 | 0.936458 |
Target: 5'- cAGGGcuccGCGUC--CGGGAACGUCGgCUAu -3' miRNA: 3'- -UCCC----UGCAGaaGUCCUUGCAGUgGGU- -5' |
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9323 | 3' | -54.7 | NC_002512.2 | + | 198043 | 0.68 | 0.936458 |
Target: 5'- aGGGGACGcggUCgucaCAGGGACGUCucgcgcguCCCc -3' miRNA: 3'- -UCCCUGC---AGaa--GUCCUUGCAGu-------GGGu -5' |
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9323 | 3' | -54.7 | NC_002512.2 | + | 181413 | 0.68 | 0.931576 |
Target: 5'- cGGGGACGcgUUCAGGAuggcCGUCAgCUg -3' miRNA: 3'- -UCCCUGCagAAGUCCUu---GCAGUgGGu -5' |
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9323 | 3' | -54.7 | NC_002512.2 | + | 190328 | 0.68 | 0.928537 |
Target: 5'- gGGGGACGacggguucgucuaccUCUacgacgaCGGGGGCGUCgGCCCGg -3' miRNA: 3'- -UCCCUGC---------------AGAa------GUCCUUGCAG-UGGGU- -5' |
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9323 | 3' | -54.7 | NC_002512.2 | + | 189981 | 0.69 | 0.909763 |
Target: 5'- cGGGaccGCGUCUaCAGGAAgGUCgACCuCAa -3' miRNA: 3'- uCCC---UGCAGAaGUCCUUgCAG-UGG-GU- -5' |
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9323 | 3' | -54.7 | NC_002512.2 | + | 225703 | 0.69 | 0.897496 |
Target: 5'- --cGGCGUCcUCAGGAuguGUCGCCCGc -3' miRNA: 3'- uccCUGCAGaAGUCCUug-CAGUGGGU- -5' |
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9323 | 3' | -54.7 | NC_002512.2 | + | 223788 | 0.7 | 0.891029 |
Target: 5'- -cGGACGUCgcCGGGGACGUCGgCg- -3' miRNA: 3'- ucCCUGCAGaaGUCCUUGCAGUgGgu -5' |
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9323 | 3' | -54.7 | NC_002512.2 | + | 94493 | 0.71 | 0.84781 |
Target: 5'- cGGGGACGcagUCgUCGGGcGCGUCuucgaacccGCCCAg -3' miRNA: 3'- -UCCCUGC---AGaAGUCCuUGCAG---------UGGGU- -5' |
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9323 | 3' | -54.7 | NC_002512.2 | + | 211566 | 0.71 | 0.84781 |
Target: 5'- cGGGAUGUCccCAugcGGGGCGUC-CCCAu -3' miRNA: 3'- uCCCUGCAGaaGU---CCUUGCAGuGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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