Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9323 | 5' | -53.7 | NC_002512.2 | + | 101287 | 0.66 | 0.986342 |
Target: 5'- --gGCAGguacccggACGUCCCGGccucucgcaGGGUcGGCCc -3' miRNA: 3'- caaCGUCa-------UGCAGGGCU---------UCUA-CCGGa -5' |
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9323 | 5' | -53.7 | NC_002512.2 | + | 182000 | 0.66 | 0.982787 |
Target: 5'- --gGCGG-ACGUCCuCGggGucaGGCCc -3' miRNA: 3'- caaCGUCaUGCAGG-GCuuCua-CCGGa -5' |
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9323 | 5' | -53.7 | NC_002512.2 | + | 75251 | 0.66 | 0.982787 |
Target: 5'- --cGCGGccGCGUCCCGuccccacGAUGGCg- -3' miRNA: 3'- caaCGUCa-UGCAGGGCuu-----CUACCGga -5' |
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9323 | 5' | -53.7 | NC_002512.2 | + | 114664 | 0.66 | 0.978575 |
Target: 5'- --aGUGGgugACGuUCCUGAAGAcgucccUGGCCUg -3' miRNA: 3'- caaCGUCa--UGC-AGGGCUUCU------ACCGGA- -5' |
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9323 | 5' | -53.7 | NC_002512.2 | + | 208801 | 0.67 | 0.973644 |
Target: 5'- --gGCGGU-CGcCCCGGAGcgGGaCCg -3' miRNA: 3'- caaCGUCAuGCaGGGCUUCuaCC-GGa -5' |
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9323 | 5' | -53.7 | NC_002512.2 | + | 97406 | 0.67 | 0.970892 |
Target: 5'- --cGCAGUuCcUCCUGggGcUGGCCa -3' miRNA: 3'- caaCGUCAuGcAGGGCuuCuACCGGa -5' |
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9323 | 5' | -53.7 | NC_002512.2 | + | 193908 | 0.67 | 0.970606 |
Target: 5'- --cGCGGUcggcggaucGCGUCCCGGAcuccgcuGAcGGCCg -3' miRNA: 3'- caaCGUCA---------UGCAGGGCUU-------CUaCCGGa -5' |
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9323 | 5' | -53.7 | NC_002512.2 | + | 198997 | 0.67 | 0.961409 |
Target: 5'- gGUUGUccgcGUACGUCUCGA---UGGCCUc -3' miRNA: 3'- -CAACGu---CAUGCAGGGCUucuACCGGA- -5' |
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9323 | 5' | -53.7 | NC_002512.2 | + | 174158 | 0.68 | 0.954011 |
Target: 5'- --cGUGGUcaccGCGUCCCGggGuacgAUGGUCg -3' miRNA: 3'- caaCGUCA----UGCAGGGCuuC----UACCGGa -5' |
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9323 | 5' | -53.7 | NC_002512.2 | + | 123620 | 0.68 | 0.94571 |
Target: 5'- -cUGCAGUAUuugaagacggGUCCCGggGAgagcGcGCCg -3' miRNA: 3'- caACGUCAUG----------CAGGGCuuCUa---C-CGGa -5' |
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9323 | 5' | -53.7 | NC_002512.2 | + | 96681 | 0.69 | 0.931513 |
Target: 5'- --gGCGGacgACGUCCCGAAGAUaacgGGUUUc -3' miRNA: 3'- caaCGUCa--UGCAGGGCUUCUA----CCGGA- -5' |
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9323 | 5' | -53.7 | NC_002512.2 | + | 157062 | 0.69 | 0.931513 |
Target: 5'- --gGCGG-GCGUCCCGggGuccggGGUCg -3' miRNA: 3'- caaCGUCaUGCAGGGCuuCua---CCGGa -5' |
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9323 | 5' | -53.7 | NC_002512.2 | + | 36837 | 0.69 | 0.926309 |
Target: 5'- --cGCGGUcagAUGUCCUGuaucuGGUGGCCa -3' miRNA: 3'- caaCGUCA---UGCAGGGCuu---CUACCGGa -5' |
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9323 | 5' | -53.7 | NC_002512.2 | + | 38918 | 0.69 | 0.923072 |
Target: 5'- --cGUAGUGCGaCCCGucagaaguuuuaucGGGUGGCCg -3' miRNA: 3'- caaCGUCAUGCaGGGCu-------------UCUACCGGa -5' |
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9323 | 5' | -53.7 | NC_002512.2 | + | 102823 | 0.69 | 0.920867 |
Target: 5'- --cGCAGaagacgcACGUCCgCGAGGAgacgGGCCa -3' miRNA: 3'- caaCGUCa------UGCAGG-GCUUCUa---CCGGa -5' |
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9323 | 5' | -53.7 | NC_002512.2 | + | 187080 | 0.7 | 0.909276 |
Target: 5'- --aGgAGgcCGUgCUGAAGAUGGCCg -3' miRNA: 3'- caaCgUCauGCAgGGCUUCUACCGGa -5' |
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9323 | 5' | -53.7 | NC_002512.2 | + | 127592 | 0.7 | 0.909276 |
Target: 5'- -aUGUAGgcguCGUCCaCGguGGUGGCCa -3' miRNA: 3'- caACGUCau--GCAGG-GCuuCUACCGGa -5' |
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9323 | 5' | -53.7 | NC_002512.2 | + | 185857 | 0.7 | 0.903129 |
Target: 5'- --aGCGGUACGUCgaGGAGGgcgccucgGGCCg -3' miRNA: 3'- caaCGUCAUGCAGggCUUCUa-------CCGGa -5' |
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9323 | 5' | -53.7 | NC_002512.2 | + | 4007 | 0.7 | 0.903129 |
Target: 5'- --aGCAGUAU-UCCCGAGGcgGaGCCg -3' miRNA: 3'- caaCGUCAUGcAGGGCUUCuaC-CGGa -5' |
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9323 | 5' | -53.7 | NC_002512.2 | + | 159933 | 0.7 | 0.890143 |
Target: 5'- -gUGCGaUACGUCCCc--GGUGGCCg -3' miRNA: 3'- caACGUcAUGCAGGGcuuCUACCGGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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