miRNA: 3'- caaCGU-CAUGCAGGGCU-----UCUACCGGa -5'
miRNA: 3'- caaCGuCauGCAGGGCUU---CUACCGGA- -5'
miRNA: 3'- cAACGUCAUgCAGGGCUuCuaCCGga -5'
miRNA: 3'- caaCGUCAUGCAGG---------------GCUUCUaCCGGA- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9323 | 5' | -53.7 | NC_002512.2 | + | 208362 | 0.71 | 0.86899 |
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Target: 5'- --aGCAgGUGCGUUUCGAcgacgAGGUGGCCc -3' miRNA: 3'- caaCGU-CAUGCAGGGCU-----UCUACCGGa -5' |
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| 9323 | 5' | -53.7 | NC_002512.2 | + | 112761 | 0.71 | 0.86899 |
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Target: 5'- --aGCcGgcCGUCCCGGAcacGGUGGCCUu -3' miRNA: 3'- caaCGuCauGCAGGGCUU---CUACCGGA- -5' |
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| 9323 | 5' | -53.7 | NC_002512.2 | + | 113710 | 0.71 | 0.837865 |
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Target: 5'- cUUGUAGUGgGUCCCGAcGcgGGCg- -3' miRNA: 3'- cAACGUCAUgCAGGGCUuCuaCCGga -5' |
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| 9323 | 5' | -53.7 | NC_002512.2 | + | 198727 | 0.73 | 0.770987 |
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Target: 5'- --gGCGGUGCGUCUcccgggccagguccgCGAAGAcGGCCUu -3' miRNA: 3'- caaCGUCAUGCAGG---------------GCUUCUaCCGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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