Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9324 | 5' | -55.5 | NC_002512.2 | + | 99231 | 0.66 | 0.969869 |
Target: 5'- cCUCG-GAGAgcccCGCGCagaagUCGAGGGa -3' miRNA: 3'- -GAGCuCUCUa---GCGCGgca--AGCUCCUa -5' |
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9324 | 5' | -55.5 | NC_002512.2 | + | 223748 | 0.66 | 0.966293 |
Target: 5'- gUCGuGGAGGUCGUGuCCGacgacagcgaggUCGAGGGa -3' miRNA: 3'- gAGC-UCUCUAGCGC-GGCa-----------AGCUCCUa -5' |
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9324 | 5' | -55.5 | NC_002512.2 | + | 109018 | 0.67 | 0.940334 |
Target: 5'- cCUCGAGGGG-CGgGCgGggaCGAGGAc -3' miRNA: 3'- -GAGCUCUCUaGCgCGgCaa-GCUCCUa -5' |
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9324 | 5' | -55.5 | NC_002512.2 | + | 220018 | 0.68 | 0.914688 |
Target: 5'- gUCGAGAGGcCGCuCCGUcUCGGGGu- -3' miRNA: 3'- gAGCUCUCUaGCGcGGCA-AGCUCCua -5' |
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9324 | 5' | -55.5 | NC_002512.2 | + | 214408 | 0.68 | 0.908878 |
Target: 5'- uUCGGGccGGAgCGCGCCGcgccCGAGGAc -3' miRNA: 3'- gAGCUC--UCUaGCGCGGCaa--GCUCCUa -5' |
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9324 | 5' | -55.5 | NC_002512.2 | + | 99120 | 0.69 | 0.876524 |
Target: 5'- gCUCcaAGAGAUCGCGCaCGgccggaucgUCGGGGGa -3' miRNA: 3'- -GAGc-UCUCUAGCGCG-GCa--------AGCUCCUa -5' |
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9324 | 5' | -55.5 | NC_002512.2 | + | 212207 | 0.69 | 0.869415 |
Target: 5'- gCUCGAcgagGAGAUCGuCGgCGUgcgCGGGGAc -3' miRNA: 3'- -GAGCU----CUCUAGC-GCgGCAa--GCUCCUa -5' |
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9324 | 5' | -55.5 | NC_002512.2 | + | 219669 | 0.7 | 0.814305 |
Target: 5'- gUCGGGAucuGggCGCGCCGccCGAGGAc -3' miRNA: 3'- gAGCUCU---CuaGCGCGGCaaGCUCCUa -5' |
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9324 | 5' | -55.5 | NC_002512.2 | + | 227317 | 0.71 | 0.797039 |
Target: 5'- --gGGGAGA-CGCGCCGgcgccCGAGGAa -3' miRNA: 3'- gagCUCUCUaGCGCGGCaa---GCUCCUa -5' |
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9324 | 5' | -55.5 | NC_002512.2 | + | 195087 | 0.73 | 0.683882 |
Target: 5'- aCUCGAGGGGUCGagGCCGa--GGGGAUu -3' miRNA: 3'- -GAGCUCUCUAGCg-CGGCaagCUCCUA- -5' |
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9324 | 5' | -55.5 | NC_002512.2 | + | 223921 | 0.76 | 0.545126 |
Target: 5'- -gCGAGAGGUCGaGCCGgaCGGGGAg -3' miRNA: 3'- gaGCUCUCUAGCgCGGCaaGCUCCUa -5' |
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9324 | 5' | -55.5 | NC_002512.2 | + | 104958 | 0.76 | 0.51624 |
Target: 5'- gUCGGGAGAUCGaCGCCGgguccgccUCGAGGu- -3' miRNA: 3'- gAGCUCUCUAGC-GCGGCa-------AGCUCCua -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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