Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9328 | 3' | -55.6 | NC_002512.2 | + | 222382 | 0.66 | 0.948651 |
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Target: 5'- cGGCGggcucugGCUCCUCAuGGAGCgccgcGGCUUc -3' miRNA: 3'- -UUGCa------CGAGGAGUuCCUCGa----CUGGAu -5' |
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| 9328 | 3' | -55.6 | NC_002512.2 | + | 91516 | 0.66 | 0.941555 |
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Target: 5'- uACGUGCacucggagccgcuggUCCUgGAGGAggaccaccuggcGCUGACCa- -3' miRNA: 3'- uUGCACG---------------AGGAgUUCCU------------CGACUGGau -5' |
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| 9328 | 3' | -55.6 | NC_002512.2 | + | 41009 | 0.66 | 0.924457 |
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Target: 5'- cGCGaUGCU-CUCGGGGAGCUGAg--- -3' miRNA: 3'- uUGC-ACGAgGAGUUCCUCGACUggau -5' |
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| 9328 | 3' | -55.6 | NC_002512.2 | + | 207902 | 0.68 | 0.873434 |
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Target: 5'- cGGCGgugGCUCCUCGAcgcGGAGCUcgaacgGGCCc- -3' miRNA: 3'- -UUGCa--CGAGGAGUU---CCUCGA------CUGGau -5' |
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| 9328 | 3' | -55.6 | NC_002512.2 | + | 124291 | 0.69 | 0.799832 |
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Target: 5'- gGGCGUGCggCUCGAccgcgcGGAGCUGAUCg- -3' miRNA: 3'- -UUGCACGagGAGUU------CCUCGACUGGau -5' |
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| 9328 | 3' | -55.6 | NC_002512.2 | + | 121020 | 0.73 | 0.622519 |
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Target: 5'- gGGCGUGCUgaCCUCGGGcGAGCgcGACCUc -3' miRNA: 3'- -UUGCACGA--GGAGUUC-CUCGa-CUGGAu -5' |
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| 9328 | 3' | -55.6 | NC_002512.2 | + | 97403 | 0.73 | 0.622519 |
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Target: 5'- uGACGcaGUUCCUCcuGGGGCUGGCCa- -3' miRNA: 3'- -UUGCa-CGAGGAGuuCCUCGACUGGau -5' |
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| 9328 | 3' | -55.6 | NC_002512.2 | + | 185528 | 0.73 | 0.59185 |
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Target: 5'- ----cGCUCUUCGAGGGGgUGGCCUAc -3' miRNA: 3'- uugcaCGAGGAGUUCCUCgACUGGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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