Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9328 | 5' | -52 | NC_002512.2 | + | 165489 | 0.66 | 0.995439 |
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Target: 5'- -uUCGGGUGGCUgcGCGUGGGgcGCu -3' miRNA: 3'- guAGCUCGUCGAgaCGCACCUaaUGc -5' |
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| 9328 | 5' | -52 | NC_002512.2 | + | 180366 | 0.67 | 0.990789 |
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Target: 5'- aGUCGcGCAgggcgccgauGCUCUGCG-GGGggACGa -3' miRNA: 3'- gUAGCuCGU----------CGAGACGCaCCUaaUGC- -5' |
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| 9328 | 5' | -52 | NC_002512.2 | + | 156330 | 0.67 | 0.989519 |
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Target: 5'- uCGUCGAGCGGCU--GCG-GGAccUGCa -3' miRNA: 3'- -GUAGCUCGUCGAgaCGCaCCUa-AUGc -5' |
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| 9328 | 5' | -52 | NC_002512.2 | + | 157156 | 0.67 | 0.988117 |
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Target: 5'- --gCGGGCGGCgggccgggCgGCGUGGGcgGCGg -3' miRNA: 3'- guaGCUCGUCGa-------GaCGCACCUaaUGC- -5' |
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| 9328 | 5' | -52 | NC_002512.2 | + | 6199 | 0.68 | 0.984879 |
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Target: 5'- -uUCGGGUAuGCUCgcuUGUGUGGGUU-CGg -3' miRNA: 3'- guAGCUCGU-CGAG---ACGCACCUAAuGC- -5' |
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| 9328 | 5' | -52 | NC_002512.2 | + | 216340 | 0.68 | 0.984702 |
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Target: 5'- gGUCG-GCGGCUCgaucuuggugaagUGCGUGG--UGCGc -3' miRNA: 3'- gUAGCuCGUCGAG-------------ACGCACCuaAUGC- -5' |
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| 9328 | 5' | -52 | NC_002512.2 | + | 175928 | 0.69 | 0.968161 |
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Target: 5'- uGUCGAGCAGggucCUCUGCGcGGuc-ACGu -3' miRNA: 3'- gUAGCUCGUC----GAGACGCaCCuaaUGC- -5' |
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| 9328 | 5' | -52 | NC_002512.2 | + | 213165 | 0.77 | 0.665899 |
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Target: 5'- --gCGGGuCAGCUCUGCGUGGucgGCa -3' miRNA: 3'- guaGCUC-GUCGAGACGCACCuaaUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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