Results 1 - 20 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9330 | 3' | -60.6 | NC_002512.2 | + | 153157 | 0.66 | 0.829597 |
Target: 5'- -cGGUCCggaaagGCgACCUacGGGGCGGCgggcagCGGUg -3' miRNA: 3'- caCCAGG------UG-UGGG--UCCUGCCGa-----GCCA- -5' |
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9330 | 3' | -60.6 | NC_002512.2 | + | 96028 | 0.66 | 0.829597 |
Target: 5'- ----gCCGC-CCCAGGACGucCUCGGUg -3' miRNA: 3'- caccaGGUGuGGGUCCUGCc-GAGCCA- -5' |
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9330 | 3' | -60.6 | NC_002512.2 | + | 184396 | 0.66 | 0.805108 |
Target: 5'- cGUGG-CCGC-CgCCGGGGgGcGCUCGGc -3' miRNA: 3'- -CACCaGGUGuG-GGUCCUgC-CGAGCCa -5' |
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9330 | 3' | -60.6 | NC_002512.2 | + | 155827 | 0.66 | 0.805108 |
Target: 5'- cGUGcagaUCCGCuCCCGGGAgGGCUUcaaGGUg -3' miRNA: 3'- -CACc---AGGUGuGGGUCCUgCCGAG---CCA- -5' |
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9330 | 3' | -60.6 | NC_002512.2 | + | 191669 | 0.66 | 0.796655 |
Target: 5'- --cGUCCGCGuCCCGGGGCugGGCgacCGGa -3' miRNA: 3'- cacCAGGUGU-GGGUCCUG--CCGa--GCCa -5' |
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9330 | 3' | -60.6 | NC_002512.2 | + | 121160 | 0.66 | 0.796655 |
Target: 5'- -cGG-CCGCgGCCCAGGGCGaggcguuCUCGGa -3' miRNA: 3'- caCCaGGUG-UGGGUCCUGCc------GAGCCa -5' |
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9330 | 3' | -60.6 | NC_002512.2 | + | 95614 | 0.66 | 0.796655 |
Target: 5'- -cGGgaccCCGCGCggacgacgaaggCCAGGggGCGGCUCGGc -3' miRNA: 3'- caCCa---GGUGUG------------GGUCC--UGCCGAGCCa -5' |
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9330 | 3' | -60.6 | NC_002512.2 | + | 108452 | 0.66 | 0.796655 |
Target: 5'- -gGGUCC-CuCCCcGGGCGGCgcCGGg -3' miRNA: 3'- caCCAGGuGuGGGuCCUGCCGa-GCCa -5' |
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9330 | 3' | -60.6 | NC_002512.2 | + | 217720 | 0.66 | 0.78807 |
Target: 5'- -aGGUCCGCuuCaGGGGCGGCagGGa -3' miRNA: 3'- caCCAGGUGugGgUCCUGCCGagCCa -5' |
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9330 | 3' | -60.6 | NC_002512.2 | + | 118857 | 0.66 | 0.779359 |
Target: 5'- aUGGUCCuggGCAUCCuGGAguaCGGC-CGGg -3' miRNA: 3'- cACCAGG---UGUGGGuCCU---GCCGaGCCa -5' |
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9330 | 3' | -60.6 | NC_002512.2 | + | 44048 | 0.66 | 0.779359 |
Target: 5'- cGUGGUCCACuuUUCGGGGCGccuagaUCGGUu -3' miRNA: 3'- -CACCAGGUGu-GGGUCCUGCcg----AGCCA- -5' |
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9330 | 3' | -60.6 | NC_002512.2 | + | 185475 | 0.66 | 0.829597 |
Target: 5'- cGUGGUCCugcCGCCgCAGGucAUGGCgcacgcCGGg -3' miRNA: 3'- -CACCAGGu--GUGG-GUCC--UGCCGa-----GCCa -5' |
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9330 | 3' | -60.6 | NC_002512.2 | + | 93028 | 0.67 | 0.761591 |
Target: 5'- uGUGGuUCCACACggaggCGGGACGGCUg--- -3' miRNA: 3'- -CACC-AGGUGUGg----GUCCUGCCGAgcca -5' |
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9330 | 3' | -60.6 | NC_002512.2 | + | 132530 | 0.67 | 0.743413 |
Target: 5'- -cGGUCCcCGCuCCGcGGGCGGCgccgUCGGc -3' miRNA: 3'- caCCAGGuGUG-GGU-CCUGCCG----AGCCa -5' |
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9330 | 3' | -60.6 | NC_002512.2 | + | 211331 | 0.67 | 0.761591 |
Target: 5'- -cGGUCCGgGCCCgggcgcuggGGGACGcGCgCGGc -3' miRNA: 3'- caCCAGGUgUGGG---------UCCUGC-CGaGCCa -5' |
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9330 | 3' | -60.6 | NC_002512.2 | + | 113219 | 0.67 | 0.770531 |
Target: 5'- -gGGUCCuCGCCCGGGGagacgGGCaCGGc -3' miRNA: 3'- caCCAGGuGUGGGUCCUg----CCGaGCCa -5' |
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9330 | 3' | -60.6 | NC_002512.2 | + | 223219 | 0.67 | 0.770531 |
Target: 5'- -cGGagCGCGuCCC-GGcCGGCUCGGUg -3' miRNA: 3'- caCCagGUGU-GGGuCCuGCCGAGCCA- -5' |
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9330 | 3' | -60.6 | NC_002512.2 | + | 157136 | 0.68 | 0.706075 |
Target: 5'- -gGGUCgGCggcggGCCCGGGGCGgGCggCGGg -3' miRNA: 3'- caCCAGgUG-----UGGGUCCUGC-CGa-GCCa -5' |
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9330 | 3' | -60.6 | NC_002512.2 | + | 204871 | 0.68 | 0.676497 |
Target: 5'- -aGGUCCGCGaCCGacggcgagcucucGGACGGCgagCGGUc -3' miRNA: 3'- caCCAGGUGUgGGU-------------CCUGCCGa--GCCA- -5' |
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9330 | 3' | -60.6 | NC_002512.2 | + | 218106 | 0.69 | 0.638868 |
Target: 5'- -cGGgcgUCGCGCCCGGGGucGCUCGGg -3' miRNA: 3'- caCCa--GGUGUGGGUCCUgcCGAGCCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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