Results 21 - 30 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9330 | 3' | -60.6 | NC_002512.2 | + | 105884 | 0.69 | 0.619516 |
Target: 5'- -gGGUCCGucuccCACCC-GGugGGCUCGc- -3' miRNA: 3'- caCCAGGU-----GUGGGuCCugCCGAGCca -5' |
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9330 | 3' | -60.6 | NC_002512.2 | + | 158617 | 0.69 | 0.619516 |
Target: 5'- -gGGUCCugGcggCCCuGGACGGCUCc-- -3' miRNA: 3'- caCCAGGugU---GGGuCCUGCCGAGcca -5' |
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9330 | 3' | -60.6 | NC_002512.2 | + | 103449 | 0.69 | 0.60985 |
Target: 5'- -cGGUCCGaGCgCGGGuccgaggacGCGGCUCGGg -3' miRNA: 3'- caCCAGGUgUGgGUCC---------UGCCGAGCCa -5' |
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9330 | 3' | -60.6 | NC_002512.2 | + | 178229 | 0.7 | 0.552376 |
Target: 5'- -aGGUCCAgGgucuCCgGGGACGGCgCGGUc -3' miRNA: 3'- caCCAGGUgU----GGgUCCUGCCGaGCCA- -5' |
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9330 | 3' | -60.6 | NC_002512.2 | + | 116437 | 0.71 | 0.533551 |
Target: 5'- aUGGUCCACcugaucgccACCCagcucgGGGACGGggCGGUg -3' miRNA: 3'- cACCAGGUG---------UGGG------UCCUGCCgaGCCA- -5' |
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9330 | 3' | -60.6 | NC_002512.2 | + | 183945 | 0.71 | 0.524224 |
Target: 5'- cUGGuUCUGCuCCCGGGACGcgcGCUCGGg -3' miRNA: 3'- cACC-AGGUGuGGGUCCUGC---CGAGCCa -5' |
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9330 | 3' | -60.6 | NC_002512.2 | + | 145032 | 0.71 | 0.505762 |
Target: 5'- -cGGgcgCgcaACGCCCGGGACGGC-CGGg -3' miRNA: 3'- caCCa--Gg--UGUGGGUCCUGCCGaGCCa -5' |
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9330 | 3' | -60.6 | NC_002512.2 | + | 204808 | 0.72 | 0.460916 |
Target: 5'- -aGGUCCGCGuCCCGGGggcgcgGCGGCUCcGUc -3' miRNA: 3'- caCCAGGUGU-GGGUCC------UGCCGAGcCA- -5' |
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9330 | 3' | -60.6 | NC_002512.2 | + | 219419 | 0.75 | 0.326381 |
Target: 5'- --cGcCCACGCCCGGGGCGGCggCGGc -3' miRNA: 3'- cacCaGGUGUGGGUCCUGCCGa-GCCa -5' |
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9330 | 3' | -60.6 | NC_002512.2 | + | 178818 | 0.77 | 0.233774 |
Target: 5'- uUGGUCCgacaGCGCCC-GGACGuGCUCGGg -3' miRNA: 3'- cACCAGG----UGUGGGuCCUGC-CGAGCCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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