Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9330 | 5' | -56.5 | NC_002512.2 | + | 162153 | 0.66 | 0.952311 |
Target: 5'- gAGGCCGGcacgCcGACCgcgaaaGCGGCcgcguccgUCGGUCa -3' miRNA: 3'- -UCUGGCCa---GaCUGGa-----UGUCG--------AGCCAG- -5' |
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9330 | 5' | -56.5 | NC_002512.2 | + | 34961 | 0.66 | 0.949918 |
Target: 5'- cGACCGGUCggcaugcGACCcaguuaucgauauucUACGcGCgaugCGGUCg -3' miRNA: 3'- uCUGGCCAGa------CUGG---------------AUGU-CGa---GCCAG- -5' |
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9330 | 5' | -56.5 | NC_002512.2 | + | 209821 | 0.66 | 0.948279 |
Target: 5'- cGGACuCGGUCUGGCC--CAGUucuucccggaUCGGcUCu -3' miRNA: 3'- -UCUG-GCCAGACUGGauGUCG----------AGCC-AG- -5' |
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9330 | 5' | -56.5 | NC_002512.2 | + | 191193 | 0.66 | 0.944028 |
Target: 5'- cGACC-GUCU--CCUccguCGGCUCGGUCu -3' miRNA: 3'- uCUGGcCAGAcuGGAu---GUCGAGCCAG- -5' |
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9330 | 5' | -56.5 | NC_002512.2 | + | 186093 | 0.66 | 0.944028 |
Target: 5'- gAGACCGGUCggcuCCUGCuGUgCGGcCc -3' miRNA: 3'- -UCUGGCCAGacu-GGAUGuCGaGCCaG- -5' |
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9330 | 5' | -56.5 | NC_002512.2 | + | 168764 | 0.67 | 0.924789 |
Target: 5'- aAGACCuGGUCguuGCCgUACGGCgacaGGUCc -3' miRNA: 3'- -UCUGG-CCAGac-UGG-AUGUCGag--CCAG- -5' |
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9330 | 5' | -56.5 | NC_002512.2 | + | 191353 | 0.67 | 0.913817 |
Target: 5'- gGGGuaGGUCgccgcgcGACCU-UGGCUCGGUCu -3' miRNA: 3'- -UCUggCCAGa------CUGGAuGUCGAGCCAG- -5' |
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9330 | 5' | -56.5 | NC_002512.2 | + | 127663 | 0.67 | 0.913817 |
Target: 5'- cGGCCGGUCgagGGCCgcucCGGCcgCGG-Cg -3' miRNA: 3'- uCUGGCCAGa--CUGGau--GUCGa-GCCaG- -5' |
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9330 | 5' | -56.5 | NC_002512.2 | + | 163825 | 0.67 | 0.90195 |
Target: 5'- aAGAUCGaGUCUGG---GCAGCUCGG-Cg -3' miRNA: 3'- -UCUGGC-CAGACUggaUGUCGAGCCaG- -5' |
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9330 | 5' | -56.5 | NC_002512.2 | + | 108525 | 0.67 | 0.895686 |
Target: 5'- cGACCGGcCgggGACCcgACGGCggagaaGGUCc -3' miRNA: 3'- uCUGGCCaGa--CUGGa-UGUCGag----CCAG- -5' |
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9330 | 5' | -56.5 | NC_002512.2 | + | 201848 | 0.67 | 0.895686 |
Target: 5'- cGGCCGGcagCUcGCCUGgGGCcugCGGUCc -3' miRNA: 3'- uCUGGCCa--GAcUGGAUgUCGa--GCCAG- -5' |
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9330 | 5' | -56.5 | NC_002512.2 | + | 222255 | 0.68 | 0.882509 |
Target: 5'- uGGCgGGUCucgugaUGGCCUGCGcccuGCUCGGg- -3' miRNA: 3'- uCUGgCCAG------ACUGGAUGU----CGAGCCag -5' |
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9330 | 5' | -56.5 | NC_002512.2 | + | 209453 | 0.69 | 0.838102 |
Target: 5'- cGGCCGG-CUGAUCUuCGGCUcCGG-Cg -3' miRNA: 3'- uCUGGCCaGACUGGAuGUCGA-GCCaG- -5' |
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9330 | 5' | -56.5 | NC_002512.2 | + | 122495 | 0.69 | 0.830046 |
Target: 5'- -cGCCGGcCUGaACCUcuCGGuCUCGGUCu -3' miRNA: 3'- ucUGGCCaGAC-UGGAu-GUC-GAGCCAG- -5' |
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9330 | 5' | -56.5 | NC_002512.2 | + | 115680 | 0.7 | 0.804889 |
Target: 5'- uGGGCuCGGgccgGGCCUACAGCaCGGUg -3' miRNA: 3'- -UCUG-GCCaga-CUGGAUGUCGaGCCAg -5' |
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9330 | 5' | -56.5 | NC_002512.2 | + | 172251 | 0.7 | 0.778404 |
Target: 5'- uGGUCGGUCUGcucGCCUcACGcccGCUCGGUCg -3' miRNA: 3'- uCUGGCCAGAC---UGGA-UGU---CGAGCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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