Results 1 - 20 of 50 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9332 | 3' | -62.1 | NC_002512.2 | + | 127382 | 0.66 | 0.784128 |
Target: 5'- aCGGCCGcGaCUCCcgCGGCGGGaaCgACGc -3' miRNA: 3'- -GCCGGC-C-GAGGa-GUCGCCCagGaUGC- -5' |
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9332 | 3' | -62.1 | NC_002512.2 | + | 114167 | 0.66 | 0.784128 |
Target: 5'- gGGcCCGGUccUCCUCGGUGGcG-CCgGCGu -3' miRNA: 3'- gCC-GGCCG--AGGAGUCGCC-CaGGaUGC- -5' |
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9332 | 3' | -62.1 | NC_002512.2 | + | 157086 | 0.66 | 0.784128 |
Target: 5'- -cGCCGGCgCCcaCGGCGGGcCCgucccgGCGa -3' miRNA: 3'- gcCGGCCGaGGa-GUCGCCCaGGa-----UGC- -5' |
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9332 | 3' | -62.1 | NC_002512.2 | + | 190080 | 0.66 | 0.775512 |
Target: 5'- gCGGCCGGU----CAGCGGGcUCggCUGCGg -3' miRNA: 3'- -GCCGGCCGaggaGUCGCCC-AG--GAUGC- -5' |
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9332 | 3' | -62.1 | NC_002512.2 | + | 144833 | 0.66 | 0.775512 |
Target: 5'- gGGCaCGGCgcugCUCAGCauGUCCUACu -3' miRNA: 3'- gCCG-GCCGag--GAGUCGccCAGGAUGc -5' |
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9332 | 3' | -62.1 | NC_002512.2 | + | 217691 | 0.66 | 0.774644 |
Target: 5'- gGGCUGGCUCUUCcccgccGGCGucgacgaGGUCCgcuucagggGCGg -3' miRNA: 3'- gCCGGCCGAGGAG------UCGC-------CCAGGa--------UGC- -5' |
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9332 | 3' | -62.1 | NC_002512.2 | + | 184557 | 0.66 | 0.766785 |
Target: 5'- gGGCCGGCggacggcCCUCcgacccGCGGG-CCggcaggGCGg -3' miRNA: 3'- gCCGGCCGa------GGAGu-----CGCCCaGGa-----UGC- -5' |
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9332 | 3' | -62.1 | NC_002512.2 | + | 181586 | 0.66 | 0.766785 |
Target: 5'- gCGG-CGGCaggggCGGCGGGUCCgcgACGu -3' miRNA: 3'- -GCCgGCCGagga-GUCGCCCAGGa--UGC- -5' |
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9332 | 3' | -62.1 | NC_002512.2 | + | 175793 | 0.66 | 0.766785 |
Target: 5'- aGGCCGaCagCUUGGCGGcGUCCcgGCGg -3' miRNA: 3'- gCCGGCcGagGAGUCGCC-CAGGa-UGC- -5' |
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9332 | 3' | -62.1 | NC_002512.2 | + | 156477 | 0.66 | 0.766785 |
Target: 5'- gGGgCGGCgggCUCGGCGGG-CUggGCGg -3' miRNA: 3'- gCCgGCCGag-GAGUCGCCCaGGa-UGC- -5' |
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9332 | 3' | -62.1 | NC_002512.2 | + | 169623 | 0.66 | 0.757955 |
Target: 5'- uCGGCCGGCaggUCCagGuGuCGGGU-CUGCGg -3' miRNA: 3'- -GCCGGCCG---AGGagU-C-GCCCAgGAUGC- -5' |
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9332 | 3' | -62.1 | NC_002512.2 | + | 197601 | 0.66 | 0.749029 |
Target: 5'- aCGaGCCGccCUCCUCAuGCGGGccgCCUAUu -3' miRNA: 3'- -GC-CGGCc-GAGGAGU-CGCCCa--GGAUGc -5' |
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9332 | 3' | -62.1 | NC_002512.2 | + | 199621 | 0.66 | 0.749029 |
Target: 5'- gGcGCCGGCUCCgcgCGuccGCGGGgacccguuUCCcGCGg -3' miRNA: 3'- gC-CGGCCGAGGa--GU---CGCCC--------AGGaUGC- -5' |
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9332 | 3' | -62.1 | NC_002512.2 | + | 125183 | 0.66 | 0.749029 |
Target: 5'- uGGCCGGCcCCUUccaGGUGGccGUCCaggACGc -3' miRNA: 3'- gCCGGCCGaGGAG---UCGCC--CAGGa--UGC- -5' |
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9332 | 3' | -62.1 | NC_002512.2 | + | 128797 | 0.66 | 0.749029 |
Target: 5'- uGGCCgGGgUCUUCAGCGagaGGUCg-GCGg -3' miRNA: 3'- gCCGG-CCgAGGAGUCGC---CCAGgaUGC- -5' |
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9332 | 3' | -62.1 | NC_002512.2 | + | 220390 | 0.66 | 0.746333 |
Target: 5'- gGGCCGGC-CgUCGucuacccgccgcucGCGGGcccgcccgCCUACGg -3' miRNA: 3'- gCCGGCCGaGgAGU--------------CGCCCa-------GGAUGC- -5' |
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9332 | 3' | -62.1 | NC_002512.2 | + | 482 | 0.66 | 0.740014 |
Target: 5'- aGGaCCGGC-CCUCAcGaCGGGUgCCUGu- -3' miRNA: 3'- gCC-GGCCGaGGAGU-C-GCCCA-GGAUgc -5' |
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9332 | 3' | -62.1 | NC_002512.2 | + | 127499 | 0.66 | 0.730917 |
Target: 5'- uCGGCUGGaaCCUCGGCGG--CCUucACGu -3' miRNA: 3'- -GCCGGCCgaGGAGUCGCCcaGGA--UGC- -5' |
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9332 | 3' | -62.1 | NC_002512.2 | + | 156437 | 0.67 | 0.721747 |
Target: 5'- gCGGCCgggcuggggGGCUCCgggggCGGCGGGggaCU-CGg -3' miRNA: 3'- -GCCGG---------CCGAGGa----GUCGCCCag-GAuGC- -5' |
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9332 | 3' | -62.1 | NC_002512.2 | + | 90456 | 0.67 | 0.721747 |
Target: 5'- gCGGCCGGg-CCagCuGCGGGUCCc-CGa -3' miRNA: 3'- -GCCGGCCgaGGa-GuCGCCCAGGauGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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