Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9332 | 5' | -60.2 | NC_002512.2 | + | 109037 | 0.66 | 0.824819 |
Target: 5'- -aCGAGGACCgggggGGCUCGGGggacgacCGGaCCCg -3' miRNA: 3'- ugGUUCCUGG-----UCGAGUCC-------GCCaGGGa -5' |
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9332 | 5' | -60.2 | NC_002512.2 | + | 110898 | 0.66 | 0.81746 |
Target: 5'- gGCgGAGG-CCAGCcgCAGGCaGUUCUg -3' miRNA: 3'- -UGgUUCCuGGUCGa-GUCCGcCAGGGa -5' |
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9332 | 5' | -60.2 | NC_002512.2 | + | 192878 | 0.66 | 0.812485 |
Target: 5'- cCCGGGGACgCAuauaagcgccgucgcGCUCcgcGGCGGUCCa- -3' miRNA: 3'- uGGUUCCUG-GU---------------CGAGu--CCGCCAGGga -5' |
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9332 | 5' | -60.2 | NC_002512.2 | + | 212912 | 0.66 | 0.809138 |
Target: 5'- cGCCGggAGGAaCGGCaucuucgaUCGGGCGG-CCCg -3' miRNA: 3'- -UGGU--UCCUgGUCG--------AGUCCGCCaGGGa -5' |
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9332 | 5' | -60.2 | NC_002512.2 | + | 183955 | 0.66 | 0.809138 |
Target: 5'- uCCcGGGACgCGcGCUCGGGCGucGUCgCCUg -3' miRNA: 3'- uGGuUCCUG-GU-CGAGUCCGC--CAG-GGA- -5' |
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9332 | 5' | -60.2 | NC_002512.2 | + | 188767 | 0.66 | 0.809138 |
Target: 5'- uCCAAGGGCgguuccggcggCGGCUCcggccucGGCGGcCCCg -3' miRNA: 3'- uGGUUCCUG-----------GUCGAGu------CCGCCaGGGa -5' |
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9332 | 5' | -60.2 | NC_002512.2 | + | 211140 | 0.66 | 0.80067 |
Target: 5'- gGCCGAGGACCugGGCgucauccaGGGCGuGcugCCCg -3' miRNA: 3'- -UGGUUCCUGG--UCGag------UCCGC-Ca--GGGa -5' |
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9332 | 5' | -60.2 | NC_002512.2 | + | 153335 | 0.66 | 0.80067 |
Target: 5'- uCCGcuGGGCCGGUcCGGGUcggcgGGUCCCg -3' miRNA: 3'- uGGUu-CCUGGUCGaGUCCG-----CCAGGGa -5' |
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9332 | 5' | -60.2 | NC_002512.2 | + | 227185 | 0.66 | 0.783325 |
Target: 5'- aGCCGGGGG--AGCggCAGGCGcUCCCg -3' miRNA: 3'- -UGGUUCCUggUCGa-GUCCGCcAGGGa -5' |
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9332 | 5' | -60.2 | NC_002512.2 | + | 109849 | 0.67 | 0.765484 |
Target: 5'- gUCGGGGACCGGCguggcGGCGGUggggaCCUa -3' miRNA: 3'- uGGUUCCUGGUCGagu--CCGCCAg----GGA- -5' |
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9332 | 5' | -60.2 | NC_002512.2 | + | 157046 | 0.67 | 0.765484 |
Target: 5'- -gCGAGGACCccGGCaaggCGGGC-GUCCCg -3' miRNA: 3'- ugGUUCCUGG--UCGa---GUCCGcCAGGGa -5' |
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9332 | 5' | -60.2 | NC_002512.2 | + | 217510 | 0.67 | 0.728572 |
Target: 5'- cCCGAaGGCgAGCUCGGGgGGUCgCg -3' miRNA: 3'- uGGUUcCUGgUCGAGUCCgCCAGgGa -5' |
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9332 | 5' | -60.2 | NC_002512.2 | + | 157078 | 0.67 | 0.728572 |
Target: 5'- uCCGGGGucGCCGGCgccCAcGGCGGgCCCg -3' miRNA: 3'- uGGUUCC--UGGUCGa--GU-CCGCCaGGGa -5' |
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9332 | 5' | -60.2 | NC_002512.2 | + | 95637 | 0.68 | 0.719133 |
Target: 5'- gGCCAGGGGgCGGCUC-GGCcGcCCCc -3' miRNA: 3'- -UGGUUCCUgGUCGAGuCCGcCaGGGa -5' |
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9332 | 5' | -60.2 | NC_002512.2 | + | 103464 | 0.68 | 0.700061 |
Target: 5'- uCCGAGGACgCGGCUCGggucucggcGGCGG-CCg- -3' miRNA: 3'- uGGUUCCUG-GUCGAGU---------CCGCCaGGga -5' |
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9332 | 5' | -60.2 | NC_002512.2 | + | 220318 | 0.68 | 0.680781 |
Target: 5'- gGCCGGGGGCCgGGCUCcGGGgGG-CCg- -3' miRNA: 3'- -UGGUUCCUGG-UCGAG-UCCgCCaGGga -5' |
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9332 | 5' | -60.2 | NC_002512.2 | + | 184579 | 0.68 | 0.680781 |
Target: 5'- cCCGcGGGCCGGCagGgcGGCGGUCCg- -3' miRNA: 3'- uGGUuCCUGGUCGagU--CCGCCAGGga -5' |
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9332 | 5' | -60.2 | NC_002512.2 | + | 116195 | 0.68 | 0.680781 |
Target: 5'- cGCUgcGGGCCAGg-CAGGCGGUCg-- -3' miRNA: 3'- -UGGuuCCUGGUCgaGUCCGCCAGgga -5' |
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9332 | 5' | -60.2 | NC_002512.2 | + | 194633 | 0.69 | 0.641842 |
Target: 5'- cGCgGGGGACCugcccGUccucuucuccgaUCGGGCGGUCCUg -3' miRNA: 3'- -UGgUUCCUGGu----CG------------AGUCCGCCAGGGa -5' |
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9332 | 5' | -60.2 | NC_002512.2 | + | 36211 | 0.69 | 0.632072 |
Target: 5'- uCCGAGGuCCGaCUCGGaGaCGGUCCCg -3' miRNA: 3'- uGGUUCCuGGUcGAGUC-C-GCCAGGGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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