Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9334 | 5' | -57.2 | NC_002512.2 | + | 183353 | 0.66 | 0.937222 |
Target: 5'- aUGGAGGUCGaGCAGAAGaaccUCGg- -3' miRNA: 3'- -ACCUCCAGCcCGUCUUCgccaAGCac -5' |
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9334 | 5' | -57.2 | NC_002512.2 | + | 227236 | 0.66 | 0.937222 |
Target: 5'- gGGAGG-CGcGGCGGAcgGGCGGgcaUCa-- -3' miRNA: 3'- aCCUCCaGC-CCGUCU--UCGCCa--AGcac -5' |
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9334 | 5' | -57.2 | NC_002512.2 | + | 221262 | 0.66 | 0.927441 |
Target: 5'- cGGAaGUCGGG-GGAcGCGGggcgCGUGg -3' miRNA: 3'- aCCUcCAGCCCgUCUuCGCCaa--GCAC- -5' |
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9334 | 5' | -57.2 | NC_002512.2 | + | 77784 | 0.66 | 0.91677 |
Target: 5'- --cGGGUCGGGCGGA--CGGUUCc-- -3' miRNA: 3'- accUCCAGCCCGUCUucGCCAAGcac -5' |
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9334 | 5' | -57.2 | NC_002512.2 | + | 228430 | 0.66 | 0.91677 |
Target: 5'- cGGGGGcgggaaCGGGgAGGAGCGGacgcgCGUa -3' miRNA: 3'- aCCUCCa-----GCCCgUCUUCGCCaa---GCAc -5' |
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9334 | 5' | -57.2 | NC_002512.2 | + | 93012 | 0.66 | 0.91677 |
Target: 5'- gUGGcGGaCGGGCAGcuGUGGUUCc-- -3' miRNA: 3'- -ACCuCCaGCCCGUCuuCGCCAAGcac -5' |
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9334 | 5' | -57.2 | NC_002512.2 | + | 219702 | 0.66 | 0.911102 |
Target: 5'- aGGAGcGUCGGcGCucggggcgcgAGAGGCGGccccCGUGc -3' miRNA: 3'- aCCUC-CAGCC-CG----------UCUUCGCCaa--GCAC- -5' |
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9334 | 5' | -57.2 | NC_002512.2 | + | 75783 | 0.66 | 0.911102 |
Target: 5'- gGGAGGaCgGGGCAGA-GCGGgggagCGg- -3' miRNA: 3'- aCCUCCaG-CCCGUCUuCGCCaa---GCac -5' |
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9334 | 5' | -57.2 | NC_002512.2 | + | 111249 | 0.67 | 0.905213 |
Target: 5'- cGGAGcGUCGGGCGGcacGCGGa----- -3' miRNA: 3'- aCCUC-CAGCCCGUCuu-CGCCaagcac -5' |
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9334 | 5' | -57.2 | NC_002512.2 | + | 224148 | 0.67 | 0.905213 |
Target: 5'- gGGAGG-CGGacGCGuGAGGCGGggCGg- -3' miRNA: 3'- aCCUCCaGCC--CGU-CUUCGCCaaGCac -5' |
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9334 | 5' | -57.2 | NC_002512.2 | + | 227104 | 0.67 | 0.905213 |
Target: 5'- cGGccGGGUCGGGCucagacggGGGAGCGGgugCa-- -3' miRNA: 3'- aCC--UCCAGCCCG--------UCUUCGCCaa-Gcac -5' |
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9334 | 5' | -57.2 | NC_002512.2 | + | 163926 | 0.67 | 0.899105 |
Target: 5'- -cGAGGUCGGGUAcGAGU--UUCGUGa -3' miRNA: 3'- acCUCCAGCCCGUcUUCGccAAGCAC- -5' |
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9334 | 5' | -57.2 | NC_002512.2 | + | 152911 | 0.67 | 0.899105 |
Target: 5'- cGGuccccGGUCGGucaGCGGGAGCGGUccccggUCGg- -3' miRNA: 3'- aCCu----CCAGCC---CGUCUUCGCCA------AGCac -5' |
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9334 | 5' | -57.2 | NC_002512.2 | + | 91103 | 0.67 | 0.892782 |
Target: 5'- aGGAGGaCGGGCccguggcgcuGGAGCaGUUCGa- -3' miRNA: 3'- aCCUCCaGCCCGu---------CUUCGcCAAGCac -5' |
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9334 | 5' | -57.2 | NC_002512.2 | + | 27999 | 0.67 | 0.8795 |
Target: 5'- aGGAGGUCGcGGUAcc-GUGGgaCGUGa -3' miRNA: 3'- aCCUCCAGC-CCGUcuuCGCCaaGCAC- -5' |
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9334 | 5' | -57.2 | NC_002512.2 | + | 217835 | 0.68 | 0.858049 |
Target: 5'- cGGGcGGUUcGGCAGAGGCGGcagCGg- -3' miRNA: 3'- aCCU-CCAGcCCGUCUUCGCCaa-GCac -5' |
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9334 | 5' | -57.2 | NC_002512.2 | + | 93778 | 0.69 | 0.826816 |
Target: 5'- gGGAGGUCGGG----AGCGGaUCGg- -3' miRNA: 3'- aCCUCCAGCCCgucuUCGCCaAGCac -5' |
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9334 | 5' | -57.2 | NC_002512.2 | + | 193710 | 0.69 | 0.810183 |
Target: 5'- aUGGAGGUCGuacGCAgGGAGCugcucgaGUUCGUGg -3' miRNA: 3'- -ACCUCCAGCc--CGU-CUUCGc------CAAGCAC- -5' |
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9334 | 5' | -57.2 | NC_002512.2 | + | 153016 | 0.69 | 0.810183 |
Target: 5'- cGGucGGGUC-GGCGGGAGCGGUccccggUCGg- -3' miRNA: 3'- aCC--UCCAGcCCGUCUUCGCCA------AGCac -5' |
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9334 | 5' | -57.2 | NC_002512.2 | + | 152966 | 0.69 | 0.810183 |
Target: 5'- cGGucGGGUC-GGCGGGAGCGGUccccggUCGg- -3' miRNA: 3'- aCC--UCCAGcCCGUCUUCGCCA------AGCac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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