Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9338 | 5' | -56.5 | NC_002512.2 | + | 211723 | 0.66 | 0.946203 |
Target: 5'- -aCGgGGAgCUGUGCCucggccuggCCCGCGa- -3' miRNA: 3'- gaGUgCCUgGACAUGGua-------GGGCGCaa -5' |
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9338 | 5' | -56.5 | NC_002512.2 | + | 33196 | 0.66 | 0.946203 |
Target: 5'- gCUgGCGGACg-GUGCCgcucGUCCCGCc-- -3' miRNA: 3'- -GAgUGCCUGgaCAUGG----UAGGGCGcaa -5' |
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9338 | 5' | -56.5 | NC_002512.2 | + | 94355 | 0.66 | 0.941833 |
Target: 5'- gCUCGUGGcGCCUGgcgGCCAUCCUcaggacguGCGUg -3' miRNA: 3'- -GAGUGCC-UGGACa--UGGUAGGG--------CGCAa -5' |
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9338 | 5' | -56.5 | NC_002512.2 | + | 128897 | 0.66 | 0.937239 |
Target: 5'- --gACGGGCCUGgcgAUCcgCCCGCc-- -3' miRNA: 3'- gagUGCCUGGACa--UGGuaGGGCGcaa -5' |
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9338 | 5' | -56.5 | NC_002512.2 | + | 134364 | 0.66 | 0.927374 |
Target: 5'- -aCACGGACCUG-ACgAUcugCCUGCGg- -3' miRNA: 3'- gaGUGCCUGGACaUGgUA---GGGCGCaa -5' |
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9338 | 5' | -56.5 | NC_002512.2 | + | 155977 | 0.66 | 0.927374 |
Target: 5'- -gCACGG-CCUGUACgucCCCGCGc- -3' miRNA: 3'- gaGUGCCuGGACAUGguaGGGCGCaa -5' |
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9338 | 5' | -56.5 | NC_002512.2 | + | 98247 | 0.67 | 0.910877 |
Target: 5'- gUCGCGGACCccGUACU-UCCCGUc-- -3' miRNA: 3'- gAGUGCCUGGa-CAUGGuAGGGCGcaa -5' |
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9338 | 5' | -56.5 | NC_002512.2 | + | 133559 | 0.67 | 0.904322 |
Target: 5'- gCUCGCGacgcACCUGUcgugcggcgaccgGCCcgCCCGCGg- -3' miRNA: 3'- -GAGUGCc---UGGACA-------------UGGuaGGGCGCaa -5' |
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9338 | 5' | -56.5 | NC_002512.2 | + | 120238 | 0.67 | 0.892368 |
Target: 5'- --gACGGGCCUGUcguggaggGCCuaCCCGCGg- -3' miRNA: 3'- gagUGCCUGGACA--------UGGuaGGGCGCaa -5' |
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9338 | 5' | -56.5 | NC_002512.2 | + | 143437 | 0.67 | 0.892368 |
Target: 5'- -aCGCGGcACCcGUcccCCGUCCCGCGc- -3' miRNA: 3'- gaGUGCC-UGGaCAu--GGUAGGGCGCaa -5' |
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9338 | 5' | -56.5 | NC_002512.2 | + | 99727 | 0.68 | 0.885761 |
Target: 5'- gUCGCGGGCCggcccgccGCCGggcCCCGCGg- -3' miRNA: 3'- gAGUGCCUGGaca-----UGGUa--GGGCGCaa -5' |
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9338 | 5' | -56.5 | NC_002512.2 | + | 218466 | 0.68 | 0.864683 |
Target: 5'- -cCGCGGACCgcgGUcaucuccUCGUCCCGCGg- -3' miRNA: 3'- gaGUGCCUGGa--CAu------GGUAGGGCGCaa -5' |
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9338 | 5' | -56.5 | NC_002512.2 | + | 96473 | 0.68 | 0.857253 |
Target: 5'- -gCGCGGACCgcgcgGCCGUCUCGCc-- -3' miRNA: 3'- gaGUGCCUGGaca--UGGUAGGGCGcaa -5' |
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9338 | 5' | -56.5 | NC_002512.2 | + | 145460 | 0.69 | 0.822352 |
Target: 5'- cCUCAacgacaagaccgaGGACCUGUucgaccCCGUCCCGCa-- -3' miRNA: 3'- -GAGUg------------CCUGGACAu-----GGUAGGGCGcaa -5' |
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9338 | 5' | -56.5 | NC_002512.2 | + | 180739 | 0.69 | 0.808842 |
Target: 5'- aUCugGuaGGCCUGUACCGcgUCCGCGa- -3' miRNA: 3'- gAGugC--CUGGACAUGGUa-GGGCGCaa -5' |
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9338 | 5' | -56.5 | NC_002512.2 | + | 149157 | 0.71 | 0.736123 |
Target: 5'- -cCGCgGGGCCgGUGCCG-CCCGCGg- -3' miRNA: 3'- gaGUG-CCUGGaCAUGGUaGGGCGCaa -5' |
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9338 | 5' | -56.5 | NC_002512.2 | + | 115085 | 0.71 | 0.707206 |
Target: 5'- -gCGCGGcGCCUG-GCCA-CCCGCGUg -3' miRNA: 3'- gaGUGCC-UGGACaUGGUaGGGCGCAa -5' |
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9338 | 5' | -56.5 | NC_002512.2 | + | 173379 | 0.79 | 0.325461 |
Target: 5'- cCUCGCGGACCUGUuacgggACCGucgUCCUGCGg- -3' miRNA: 3'- -GAGUGCCUGGACA------UGGU---AGGGCGCaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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