Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9339 | 5' | -55.1 | NC_002512.2 | + | 176431 | 0.66 | 0.980046 |
Target: 5'- aGGGUGGccAUCUGCgccgCGgucGGggUGUCg -3' miRNA: 3'- gCCCGCCa-UGGACGa---GC---UCuuACAGg -5' |
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9339 | 5' | -55.1 | NC_002512.2 | + | 208562 | 0.66 | 0.979406 |
Target: 5'- aGGGCGGUGCgucagGC-CGAGucgacgcgcagcggGUCCg -3' miRNA: 3'- gCCCGCCAUGga---CGaGCUCuua-----------CAGG- -5' |
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9339 | 5' | -55.1 | NC_002512.2 | + | 39338 | 0.66 | 0.977854 |
Target: 5'- aCGcGGCGGUACgCgugGcCUCaGGAGaGUCCg -3' miRNA: 3'- -GC-CCGCCAUG-Ga--C-GAGcUCUUaCAGG- -5' |
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9339 | 5' | -55.1 | NC_002512.2 | + | 207931 | 0.66 | 0.972944 |
Target: 5'- aCGGGCccGGUGg-UGCUCGGGuccGUCCu -3' miRNA: 3'- -GCCCG--CCAUggACGAGCUCuuaCAGG- -5' |
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9339 | 5' | -55.1 | NC_002512.2 | + | 128864 | 0.66 | 0.972944 |
Target: 5'- gCGGGCccuGGUgcugcGCCUGCUgagggaCGAGAcgGgCCu -3' miRNA: 3'- -GCCCG---CCA-----UGGACGA------GCUCUuaCaGG- -5' |
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9339 | 5' | -55.1 | NC_002512.2 | + | 217632 | 0.66 | 0.972679 |
Target: 5'- aCGGGUcucgugcugaugcGGaugGCCUGCUgGaAGAAcGUCCu -3' miRNA: 3'- -GCCCG-------------CCa--UGGACGAgC-UCUUaCAGG- -5' |
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9339 | 5' | -55.1 | NC_002512.2 | + | 171828 | 0.66 | 0.970214 |
Target: 5'- cCGGcaucCGGUACCg---CGGGAcgGUCCg -3' miRNA: 3'- -GCCc---GCCAUGGacgaGCUCUuaCAGG- -5' |
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9339 | 5' | -55.1 | NC_002512.2 | + | 206210 | 0.67 | 0.964173 |
Target: 5'- aGcGGCGGUGCUgggGCUUGaAGuaccgcagGUCCa -3' miRNA: 3'- gC-CCGCCAUGGa--CGAGC-UCuua-----CAGG- -5' |
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9339 | 5' | -55.1 | NC_002512.2 | + | 109027 | 0.67 | 0.964173 |
Target: 5'- gCGGGCGGggacgaggACCggggggGCUCGGGGGacgaCCg -3' miRNA: 3'- -GCCCGCCa-------UGGa-----CGAGCUCUUaca-GG- -5' |
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9339 | 5' | -55.1 | NC_002512.2 | + | 164614 | 0.67 | 0.964173 |
Target: 5'- aGGGCGuUGCaggauCUCGAGGA-GUCCg -3' miRNA: 3'- gCCCGCcAUGgac--GAGCUCUUaCAGG- -5' |
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9339 | 5' | -55.1 | NC_002512.2 | + | 196659 | 0.67 | 0.964173 |
Target: 5'- gGGGcCGGUACgUGUgcuguugCGAGGA-GUUCg -3' miRNA: 3'- gCCC-GCCAUGgACGa------GCUCUUaCAGG- -5' |
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9339 | 5' | -55.1 | NC_002512.2 | + | 221402 | 0.67 | 0.949629 |
Target: 5'- gCGGGCGGggag-GCggCGGGucUGUCCg -3' miRNA: 3'- -GCCCGCCauggaCGa-GCUCuuACAGG- -5' |
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9339 | 5' | -55.1 | NC_002512.2 | + | 121830 | 0.68 | 0.945456 |
Target: 5'- gGGGCGGgugACCUGacgaUCGcg---GUCCg -3' miRNA: 3'- gCCCGCCa--UGGACg---AGCucuuaCAGG- -5' |
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9339 | 5' | -55.1 | NC_002512.2 | + | 163653 | 0.68 | 0.945456 |
Target: 5'- aCGGGCGGcccgucaccGCCaGCUggcCGGGGAcGUCCu -3' miRNA: 3'- -GCCCGCCa--------UGGaCGA---GCUCUUaCAGG- -5' |
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9339 | 5' | -55.1 | NC_002512.2 | + | 213010 | 0.68 | 0.941063 |
Target: 5'- uCGGGCccgGGgcCCU-CUCGAGGugcGUCCg -3' miRNA: 3'- -GCCCG---CCauGGAcGAGCUCUua-CAGG- -5' |
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9339 | 5' | -55.1 | NC_002512.2 | + | 91261 | 0.68 | 0.931607 |
Target: 5'- gGGGUGGUcccCCUGUUCGcGAAUGa-- -3' miRNA: 3'- gCCCGCCAu--GGACGAGCuCUUACagg -5' |
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9339 | 5' | -55.1 | NC_002512.2 | + | 194663 | 0.68 | 0.931607 |
Target: 5'- uCGGGCGGU-CCUGCacgcccacgUCGAccugGUCUa -3' miRNA: 3'- -GCCCGCCAuGGACG---------AGCUcuuaCAGG- -5' |
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9339 | 5' | -55.1 | NC_002512.2 | + | 189208 | 0.68 | 0.921253 |
Target: 5'- gCGGGCGGca--UGCUCGAcgucGGAUccGUCCg -3' miRNA: 3'- -GCCCGCCauggACGAGCU----CUUA--CAGG- -5' |
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9339 | 5' | -55.1 | NC_002512.2 | + | 113728 | 0.69 | 0.915738 |
Target: 5'- gCGGGCGGcgAUCUGCgggcCGAGGugcgcgGUCa -3' miRNA: 3'- -GCCCGCCa-UGGACGa---GCUCUua----CAGg -5' |
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9339 | 5' | -55.1 | NC_002512.2 | + | 77788 | 0.69 | 0.915738 |
Target: 5'- uCGGGCGGacgguuCCU--UCGAGugcGUGUCCg -3' miRNA: 3'- -GCCCGCCau----GGAcgAGCUCu--UACAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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