Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9341 | 5' | -51.8 | NC_002512.2 | + | 117685 | 0.66 | 0.997538 |
Target: 5'- aCGGGUcgacGAgcugCGUGGcgcaccugcGGCgGGUGCGCAGc -3' miRNA: 3'- -GCCUA----CUa---GCACU---------UCGgCUACGUGUC- -5' |
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9341 | 5' | -51.8 | NC_002512.2 | + | 95251 | 0.66 | 0.997538 |
Target: 5'- aCGGc--GUUGUGggGCCaggGCACGGa -3' miRNA: 3'- -GCCuacUAGCACuuCGGcuaCGUGUC- -5' |
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9341 | 5' | -51.8 | NC_002512.2 | + | 224151 | 0.66 | 0.997097 |
Target: 5'- aGGcgGAcgCGUGAGGCgGGgcgggggcGCGCGGa -3' miRNA: 3'- gCCuaCUa-GCACUUCGgCUa-------CGUGUC- -5' |
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9341 | 5' | -51.8 | NC_002512.2 | + | 168811 | 0.66 | 0.996592 |
Target: 5'- gCGGugcGAUCG-GAAGUCGAaGgGCAGg -3' miRNA: 3'- -GCCua-CUAGCaCUUCGGCUaCgUGUC- -5' |
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9341 | 5' | -51.8 | NC_002512.2 | + | 98501 | 0.66 | 0.996256 |
Target: 5'- cCGGGUGAacagCGggucGAGGUgaaucggggugacgaCGAUGCACAGc -3' miRNA: 3'- -GCCUACUa---GCa---CUUCG---------------GCUACGUGUC- -5' |
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9341 | 5' | -51.8 | NC_002512.2 | + | 115289 | 0.66 | 0.996017 |
Target: 5'- gGGGUGAUCGUGAgcacgcAGaCGGUGaucCAGa -3' miRNA: 3'- gCCUACUAGCACU------UCgGCUACgu-GUC- -5' |
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9341 | 5' | -51.8 | NC_002512.2 | + | 89361 | 0.66 | 0.995296 |
Target: 5'- gCGGAgauguucUGAUCgGUGAAcugcgaguaguuGCCGAUGCGggcCAGg -3' miRNA: 3'- -GCCU-------ACUAG-CACUU------------CGGCUACGU---GUC- -5' |
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9341 | 5' | -51.8 | NC_002512.2 | + | 149501 | 0.66 | 0.994629 |
Target: 5'- gCGGGUGGUCaaaAGGCCGcgGC-CGGa -3' miRNA: 3'- -GCCUACUAGcacUUCGGCuaCGuGUC- -5' |
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9341 | 5' | -51.8 | NC_002512.2 | + | 220597 | 0.68 | 0.984761 |
Target: 5'- gGGAUccGGUCGUGcgcGAGCUGGUGCugugcCGGg -3' miRNA: 3'- gCCUA--CUAGCAC---UUCGGCUACGu----GUC- -5' |
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9341 | 5' | -51.8 | NC_002512.2 | + | 152858 | 0.76 | 0.727644 |
Target: 5'- aGGcgGAaCGUGcAGCCGGUGUGCGGg -3' miRNA: 3'- gCCuaCUaGCACuUCGGCUACGUGUC- -5' |
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9341 | 5' | -51.8 | NC_002512.2 | + | 91696 | 0.8 | 0.535444 |
Target: 5'- aGGcgGAUCGUGGAGCUGAUGgagaaGCGGg -3' miRNA: 3'- gCCuaCUAGCACUUCGGCUACg----UGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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